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dc.contributor.advisorMabusela, Wilfred T.
dc.contributor.authorYalo, Masande Nicholas
dc.date.accessioned2018-08-29T09:00:06Z
dc.date.available2018-08-29T09:00:06Z
dc.date.issued2017
dc.identifier.urihttp://hdl.handle.net/11394/6354
dc.descriptionMagister Scientiae - MSc (Chemistry)
dc.description.abstractPlants have been widely used in traditional medicine for a number of ailments, among which may be included infectious diseases such as colds, influenza, chicken pox, TB, etc. as well as lifestyle diseases such as diabetes and cancer. Seaweeds have also been shown to contribute to the maintenance of health through their nutritional and medicinal properties and recently, a great deal of interest has developed towards the isolation of bioactive compounds from marine sources due to their numerous health benefits. Furthermore, marine algae are valuable sources of structurally diverse metabolites with scientifically proven therapeutic claims. Chemical constituents of red seaweed, Porphyra capensis was investigated in this present study along with subsequent brine shrimp lethality assay analysis of the crude extracts. The compounds isolated from the plant were from the hexane (6) and butanol (2) extracts. These compounds were all isolated and purified by various chromatographic techniques, namely silica gel chromatography, Sephadex LH-20 gel as well as C18 reversed phase silica gel. The structures of the isolated compounds were analysed and characterised by NMR, GC-MS, ESI MS and FTIR spectroscopy. Eight compounds were isolated and identified as phytol, desmosterol, 9-eicosenoic acid, 5,8,11,14,17-eicosapentanoic acid, palmitic acid, methyl (E)-hexadec-9-enoate, glycerol and compound 1 (novel compound). All the compounds were isolated from Porphyra capensis for the first time. The hexane, butanol and methanol extracts were found to be non-toxic with the brine shrimp test LC50 value at least two times greater than ?g/ml.
dc.language.isoen
dc.publisherUniversity of the Western Cape
dc.subjectRed algae
dc.subjectPorphyra capensis
dc.subjectBrine shrimp lethality assay
dc.subjectChromatographic techniques
dc.subjectSephadex LH-20
dc.subjectC18 reversed
dc.titleAn investigation of the natural products composition of Porphyra capensis (a red seaweed)
dc.rights.holderUniversity of the Western Cape


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