Show simple item record

dc.contributor.advisorFisher, David
dc.contributor.authorZabida, Omer Saleh
dc.date.accessioned2019-05-09T12:08:37Z
dc.date.available2019-05-09T12:08:37Z
dc.date.issued2018
dc.identifier.urihttp://hdl.handle.net/11394/6775
dc.description>Magister Scientiae - MScen_US
dc.description.abstractIntroduction The blood-testis barrier (BTB) is formed by tight junctions between adjacent Sertoli cells. The barrier formed by these tight junction helps to create a specialized environment for spermatogenesis and provide an immunological barrier to protect developing germ cells. Methamphetamine (Meth) is known as neurotoxin however, its effects on the male reproductive system, especially on Sertoli cells and, the BTB are not well established. Therefore, this study aimed to determine the effects of Meth on the TM4 mouse testis Sertoli cell line and on the integrity of the BTB permeability. Materials and Methods This study investigated the effect of selected concentrations of Meth (0.1 μM, 1 μM, 10 μM, 20 μM and 100 μM) on TM4 mouse testis Sertoli cell line for 24 until 96 hours, using two treatments: an “acute” study (24 hrs exposure) and a “chronic” study, where treatment occurred on a daily basis over 96 hrs. The following parameters were investigated: viability, cell proliferation, mitochondrial activity, monolayer permeability.en_US
dc.language.isoenen_US
dc.publisherUniversity of the Western Capeen_US
dc.subjectBlood-testis barrier (BTB)en_US
dc.subjectMethamphetamineen_US
dc.subjectMale reproductive systemen_US
dc.subjectSertoli cellsen_US
dc.subjectCell viabilityen_US
dc.titleThe effect of methamphetamine on the blood-testis barrieren_US
dc.rights.holderUniversity of the Western Capeen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record