Magister Scientiae - MSc (Pharmaceutical Chemistry)
http://hdl.handle.net/11394/5929
2024-03-29T09:01:00ZDiscovery of heat shock protein90 inhibitors using structured-based virtual screening
http://hdl.handle.net/11394/10679
Discovery of heat shock protein90 inhibitors using structured-based virtual screening
Ngcagawule, Siyasanga
Alzheimer's disease (AD) is the most common irreversible dementia, caused by accumulation of protein aggregates in the central nervous system, with symptoms that include memory loss and behavioural abnormalities. Cholinesterase inhibitors (ChEIs) and N-methyl-D-aspartate receptor (NMDAR) antagonists are current treatment options for mild to moderate AD. These agents are not used to cure the illness, but rather as symptomatic therapy. Heat Shock Protein (HSP90), is one of the molecular chaperones that plays a role in AD pathogenesis. HSP90’s main function is to regulate the heat shock factor-1 (HSF-1) transcription factor, which is the key regulator of the heat shock response. Inhibition of HSP90 activates HSF-1 and the subsequent induction of heat shock proteins such as HSP70, HSP40 and HSP27. Such protein assist the folding of newly synthesized or misfolded proteins, preventing their aggregation. Therefore, HSP90 inhibitors protect against protein toxicity and reduces brain aggregate to form in AD. The HSP90 protein crystal structure (PDB ID; 2bz5) was used to dock a diverse structural database of about 12 000 amine containing compounds from the Maybridge Screening Collection using Fast Rigid Exhaustive Docking (FRED). The top five hundred-docked compounds were viewed using Visualization & Communication of Modeling Results (VIDA), and the top 50 compounds were analysed for important binding interactions and low Chemgausse4 scores. Ten of these compounds were selected based on their interaction profile with the desired HSP90 active site amino acids. Thereafter, the ten MayBridge compounds were drawn on Sigma-Aldrich and searched for structures that show at least 70% similarity, to the selected MayBridge compounds.
>Magister Scientiae - MSc
2023-01-01T00:00:00ZThe implementation of the African union model law on medical products regulation and the establishment of the African medicines agency
http://hdl.handle.net/11394/9651
The implementation of the African union model law on medical products regulation and the establishment of the African medicines agency
Ncube, Bakani Mark
Within Africa, there is insufficient access to quality, safe, efficacious and
affordable medical products which can partly be attributed to lack of robust
regulatory systems, a lack of competent regulatory professionals in national
medicines regulatory authorities (NMRAs) and ineffective regional
collaborations among NMRAs. In response to national regulatory challenges, a
number of regional harmonisation efforts were introduced through the African
Medicines Regulatory Harmonisation (AMRH) initiative to, among others,
expedite market authorisation of medical products and to facilitate the alignment
of national legislative frameworks with the African Union (AU) Model Law on
Medical Products Regulation.
>Magister Scientiae - MSc
2022-01-01T00:00:00ZAssessing the influence of electronic media on student perceptions towards antibiotic resistance
http://hdl.handle.net/11394/9650
Assessing the influence of electronic media on student perceptions towards antibiotic resistance
Munyayi, Prudence Vimbai
Antimicrobial resistance (AMR) and the reduced number of new antibiotic
discoveries impose a major threat to the quality of human life and effective
treatment options. Antibiotic resistant infections require prolonged hospital stays
and treatment periods, that depend on more expensive regimens. The lack of
knowledge and negative attitude among the public about antibiotics and antibiotic
resistance has led to inappropriate antibiotic use.
>Magister Scientiae - MSc
2022-01-01T00:00:00ZA comparison between estimated renal function from point-of care derived cystatin c measurements and serum creatinine derived measurements
http://hdl.handle.net/11394/9644
A comparison between estimated renal function from point-of care derived cystatin c measurements and serum creatinine derived measurements
Mogakane, Khomotso Lesedi
Serum Creatinine (SCr) is the most commonly used and cost effective
biomarker used to quantify estimated glomerular filtration rate. However, creatinine is
affected by anthropometric parameters such as muscle mass, age and gender. Cystatin-C
(CysC) is a low molecular weight protein which is freely filtered through the
glomerulus. It has been said to be as accurate as plasma creatinine and is independent of
limitations derived from anthropometric parameters. CysC can also be determined
through point of care devices which do not require the phlebotomy or experienced
laboratory personnel for its use. There have also been correlations noted in CysC serum
concentrations and cardiovascular risk assessment.
>Magister Scientiae - MSc
2022-01-01T00:00:00Z