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dc.contributor.advisorKeyster, Marshall
dc.contributor.authorGcanga, Esihle
dc.date.accessioned2020-10-15T10:47:33Z
dc.date.available2020-10-15T10:47:33Z
dc.date.issued2020
dc.identifier.urihttp://hdl.handle.net/11394/7364
dc.descriptionMasters of Scienceen_US
dc.description.abstractIn this study, we observed that phosphate (P) deficiency stunted plant growth and produced plants with poor morphological characteristics (yellow and small leaves). Furthermore, we treated plants with 0.8 mM (control) and 0.02 mM P (deficient) in addition to 6 μM methylglyoxal (MG) and we observed that the plants treated with MG had a higher germination, and better morphological characteristics (the leaves were more dark green and bigger in size) compared to the P deficient plants. However, we also observed that the P deficient plants treated with MG had low levels of both O2- and H2O2 and this could be a possible reason for the improved growth and morphological characteristics. In contrast, the P deficient plants not treated with MG had high levels of O2- and H2O2 which could be the possible reason for the observed cell death. We also performed biochemical assays including superoxide dismutase, ascorbate peroxidase, malondialdehyde content, ascorbic acid content, catalase, and most of the assays showed high levels of reactive oxygen species (ROS) and low levels of antioxidant activities in plants not treated with MG while high levels of antioxidant activities and low levels of ROS were observed in plants treated with exogenous MG. Since nitric oxide (NO) is also known to be a signalling molecule, we did a NO assay and observed that NO content increased under low exogenous doses of MG. From our findings we came to a hypothesis that MG modulates P deficiency stress in P. vulgaris through NO signalling or it might be that NO and MG work in tandem to modulate signalling pathways under P deficiency. Finally, we looked at the nutrient profile and the results showed that while there was a poor nutrient profile generally under P deficiency, there was an improvement in nutrient profile when MG was administered at low doses.en_US
dc.language.isoenen_US
dc.publisherUWCen_US
dc.subjectAntioxidant enzymesen_US
dc.subjectAscorbate peroxidaseen_US
dc.subjectBiomassen_US
dc.subjectCell deathen_US
dc.subjectHeavy metalen_US
dc.subjectHydrogen peroxideen_US
dc.subjectLipid peroxidationen_US
dc.subjectMethylglyoxalen_US
dc.subjectNitric oxideen_US
dc.subjectP. vulgarisen_US
dc.subjectPhosphate deficiencyen_US
dc.subjectReactive oxygen speciesen_US
dc.subjectSuperoxide dismutaseen_US
dc.subjectSuperoxideen_US
dc.titleMethylgyoxal signalling in Phaseolus vulgaris under phosphate deficiencyen_US
dc.rights.holderUWCen_US


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