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Cloning, expression and characterisation of Amidase Genes from a psychrotolerant Nesterenkonia isolate
(University of the Western Cape, 2009)
A nitrile and amide hydrolysing Nesterenkonia sp. was isolated from Antarctic soil and was characterised as a psychrotolerant, halotolerant and alkaliphilic extremophile. Amidases are widely distributed in both prokaryotic ...
Solution structure of the RING finger domain from the human splicing-associated protein RBBP6 using heteronuclear Nuclear Magnetic Resonance (NMR) spectroscopy
(2009)
Retinoblastoma-binding protein 6 (RBBP6) is a multi-domain human protein known to play a role in mRNA splicing, cell cycle control and apoptosis. The protein interacts with tumour suppressor proteins p53 and pRb and recent ...
A yeast 2-hybrid screen to identify and characterize interaction partners of the cancer associated protein retinoblastoma binding protein 6
(2009)
Retinoblastoma binding protein 6 (RBBP6) is a 250 kDa protein that is
implicated in mRNA processing and ubiquitination functions and has been
shown to be highly up-regulated in a number of cancers. In humans and mice,RBBP6 ...
Molecular detection and characterisation of RNA viruses of honeybees
(University of the Western Cape, 2009)
Propagation methods for honeybee viruses have not changed since these viruses were discovered. There are no suitable cell lines or cell culture techniques available for honeybee viruses. Honeybee viruses have to be manually ...
Transcription regulation and candidate diagnostic markers of esophageal cancer
(University of the Western Cape, 2009)
Esophageal cancer (EC) ranks among the ten most frequent cancers worldwide. Mortality rates associated with EC are very similar to the incidence rates due to the relatively late stage of diagnosis and the poor efficacy of ...
A proteomic analysis of drought and salt stress responsive proteins of different sorghum varieties
(University of the Western Cape, 2009)
This study reports on a proteomic analysis of sorghum proteomes in response to salt and hyperosmotic stresses. Two-dimensional gel electrophoresis (2DE) in combination with mass spectrometry (MS) was used to separate, ...
The identification of candidate genes using cDNA microarray and the analysis of two SNPs of the reelin gene in a South African austistic population
(University of the Western Cape, 2009)
Autism is a pervasive developmental disorder (PDD) that's incidence is approximately 1 in 158. It is four times more prevalent in males than females and is believed to be caused by both genetic and environmental factors. ...
Characterisation of AtPNP-A - a novel arabidopsis thaliana gene with role in water and salt homeostasis
(University of the Western Cape, 2009)
Plant natriuretic peptides (PNPs) are a novel class of extracellular, systemically mobile molecules that elicit a number of plant responses important in homeostasis and growth. Natriuretic peptides were first identified ...
Characterisation of eight non-codis Ministrs in four South African populations to aid the analysis of degraded DNA
(University of the Western Cape, 2009)
In many forensic cases, such as mass disasters reconstruction cases, the recovered DNA is highly degraded. In such incidences, typing of STR loci has become one of the most powerful tools for retrieving information from ...
Screening for subtate tolerant Geobacillus pallidus RAPc8 nitrile hydratase
(University of the Western Cape, 2009)
In this study G. pallidus RAPc8 NHase mutants were screened for reduced substrate inhibition compared to the wild type enzyme. Wild type and mutant enzymes were expressed and purified using hydrophobic interaction ...