Browsing Magister Scientiae - MSc (Biotechnology) by Title
Now showing items 44-63 of 261
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A comparative ancestry analysis of Y-chromosome DNA haplogroups using high resolution melting
(University of the Western Cape, 2018)The objective of this study is to deduce paternal ancestry using ancestry informative single nucleotide polymorphisms (SNPs) by means of High Resolution Melting (HRM). This was completed by producing a multiplex system that ... -
Comparative molecular, physiological and proteomic analyses of maize and sorghum subjected to water deficit stress
(University of the Western Cape, 2019)Drought is a major abiotic stress which causes not only differences between the mean yield and the potential yield but also yield variation from year to year. Although selection for genotypes with improved productivity ... -
A comparative proteomic analysis of two contrasting Salvia hispanica L. genotypes under salinity stress
(University of the Western cape, 2016)Salvia hispanica L. is an annual pseudocereal food crop, locally known as chia that has the ability to grow in water stress environments. The importance of chia dates back to the pre-columbian era where it was consumed as ... -
Comparison of multi-gene integration strategies in CRISPR-based transformation of Saccharomyces cerevisiae
(University of Western Cape, 2021)Saccharomyces cerevisiae is an important host in industrial biotechnology. This yeast is the host of choice for the first and second-generation biofuels for ethanol production. Genome modification in S. cerevisiae has been ... -
Comparison of multi-gene integration strategies in CRISPR-based transformation of Saccharomyces cerevisiae
(University of the Western Cape, 2021)Saccharomyces cerevisiae is an important host in industrial biotechnology. This yeast is the host of choice for the first and second-generation biofuels for ethanol production. Genome modification in S. cerevisiae has been ... -
Comparison of plasmids from clinical Lactobacillus strains
(University of the Western Cape, 2018)The vaginal mucosa is dominated by Gram positive, rod shaped lactobacilli which serve as a natural barrier against infection. In both healthy and BV infected women Lactobacillus crispatus and Lactobacillus jensennii has ... -
Comparison of plasmids from clinical Lactobacillus strains
(University of the Western Cape, 2018)The vaginal mucosa is dominated by Gram positive, rod shaped lactobacilli which serve as a natural barrier against infection. In both healthy and BV infected women Lactobacillus crispatus and Lactobacillus jensennii has ... -
Deciphering a potential cytoprotective role of novel heat shock responsive proteins using a proteomic approach
(University of the Western Cape, 2011)Myocardial infarction, commonly known as a heart attack, is a condition where the blood supply to the heart tissue is cut off, starving the tissue from oxygen and nutrient supply, with consequent lethal damage to the heart ... -
The degradation of the endocrine disrupting chemical, bisphenol-A : a comparative study between fungal and bacterial laccases
(University of the Western Cape, 2015)The degradation of endocrine disrupting chemicals (EDCs) is a topic of high importance and one that research efforts are continually being focused on. These harmful chemicals are known to cause adverse health effects in ... -
Developing a generic hazard analysis critical control point (HACCP) system for the wheat milling industry
(University of the Western Cape, 2005)In South Africa there is a need within the milling industry for controlling food safety especially due to customer's demands and government's regulations. The best way to ensure food safety is with the implementation of a ... -
Developing a lateral flow device for the detection of novel breast cancer biomarkers
(University of the Western Cape, 2023)Breast cancer is the most prevalent malignancy and one of the leading causes of death in women worldwide. Delayed diagnosis of breast cancer greatly reduces the effectiveness of treatment and is considered one of the major ... -
Developing a quantum dot nanotracker for endophytic bacteria translocation in plants
(University of the Western Cape, 2017)Endophytes are bacteria that have been shown in previous studies to promote plant growth without eliciting any sign of infection or plant disease, as well as promote bio-fortification and has thus gained much attention ... -
Development and validation of a pharmacogenomics profiling panel suitable for personalizing metformin therapy
(University of the Western Cape, 2019)The burden of non-communicable diseases (NCDs) in South Africa is predicted to increase substantially in the next decades if the necessary preventative measures are not taken. The two most common NCDs associated with ... -
Development and validation of a pharmacogenomics profiling panel suitable for personalizing Metformin therapy
(University of the Western Cape, 2019)The burden of non-communicable diseases (NCDs) in South Africa is predicted to increase substantially in the next decades if the necessary preventative measures are not taken. The two most common NCDs associated with rapid ... -
Development and validation of Non-CODIS miniSTR genotyping systems suitable for forensic case work in South Africa
(University of the Western Cape, 2010)The objective of this study was to develop and validate a six Non-CODIS miniSTR genotyping system and to determine its suitability for forensic casework in South Africa. In Non-CODIS miniSTR genotyping systems, smaller PCR ... -
Development of a COMT PCR multiplex to investigate resilience, anxiety and childhood trauma in a South African population
(University of the Western Cape, 2020)Anxiety, resilience and childhood trauma can be categorized as functional behavioural categories, with a wealth of research behind each. The research approach adopted for each, in most cases, is either from a genetic or ... -
Development of a plum chromosome doubling method and proteomics and biochemical characterization
(University of the Western Cape, 2015)Chromosome doubling has become an important tool in breeding programmes as it offers the ability of introducing novel traits into existing plants. Doubled haploid plants are highly valued by both consumers and breeders as ... -
Development of a reporter system for the analysis of xylophilus ampelinus type III secreted effectors
(University of the Western Cape, 2014)Xylophilus ampelinus, the causal agent of bacterial blight and canker of grapevines, has long been a threat to the table grape industry in the Western Cape, leading to severe economic losses due to the reduced productivity ... -
Development of an actinobacteria based in vitro transcription and translation systems
(University of the Western Cape, 2015)Heterologous metagenomic screening strategies have relied largely on the construction of DNA libraries and screening in Escherichia coli to access novel enzymes. There is an increased demand for the identification of novel ... -
Development of an aptamer based lateral flow test strip for a diabetes biomarker
(University of the Western Cape, 2019)Diabetes mellitus is one of the major chronic diseases that poses a significant risk to human health globally. Current diagnostics for diabetes are based on blood glucose measurements, which can go unnoticed for years. ...