Browsing Magister Scientiae - MSc (Biotechnology) by Issue Date
Now showing items 21-40 of 262
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Monitoring of heavy metals in the bottelary river using typha capensis and phragmites australis
(2005)The aim of this study was to use plants to determine the degree of heavy metal contamination in water and sediments in order to effectively monitor and provide possible recommendation to improve the water quality in the ... -
Analysis of ammonia-oxidizing bacteria associated with the roots of Proteaceae plant species in soils of Fynbos ecosystem
(University of the Western Cape, 2005)Molecular methods were used to investigate the microbial diversity and community structure of ammonia-oxidizing bacteria (AOB) associated with the roots of the Proteaceae plant family. The identification of ammonia ... -
Microbiology of fly ash-acid mine drainage co-disposal processes
(University of the Western Cape, 2005)The waste products acid mine drainage (AMD), formed during coal mining and fly ash (FA) from coal burning power generation, pose substantial environmental and economic problems for South Africa. Eskom has developed a ... -
The feasibility of high resolution, three-dimensional reconstruction of metal-coated surfaces in structural biology
(2006)Life is an emergent property of a complex network of interacting cellular-machines. Three-dimensional (3D), cellular structure captured at supra-atomic resolution has the potential to revolutionise our understanding of the ... -
Sample preparation methods and molecular based detection for the rapid isolation and identification of Listeria monocytogenes in food samples
(University of the Western Cape, 2006)Listeria monocytogenes is a Gram-positive bacterium responsible for listeriosis, a food-borne disease, which may result in severe illness and possible death. The importance of L. monocytogenes as a food-borne pathogen has ... -
Systematic approach to protein crystallization: emphasis on Vaccinia virus complement control protein (VCP)
(University of the Western Cape, 2006)This work examined the systematic approach to protein crystallization, exploring some of the techniques that have been developed to enhance the success rate of crystallization. The work was centered on two proteins; namely ... -
The structure of the nitrilase from Rhodococcus Rhodochrous J1: homology modeling and three-dimensional reconstruction
(University of the Western Cape, 2006)The nitrilases are an important class of industrial enzymes that are found in all phyla. These enzymes are expressed widely in prokaryotes and eukaryotes. Nitrilases convert nitriles to corresponding acids and ammonia. ... -
Screening extracts of indigenous South African plants for the presence of anti-cancer compounds
(University of the Western Cape, 2006)Early man dabbled with the use of plant extracts to cure ailments. This practice has been passed down from generation to generation and today more than 50% of the world'sdrugs are natural products or derivatives thereof. ... -
Phylogenetic diversity of nifH genes in Marion Island soil
(University of the Western Cape, 2006)The microbial life of sub-Antarctic islands plays a key role in the islands ecosystem, with microbial activities providing the majority of nutrients available for primary production. Knowledge of microbial diversity is ... -
Investigation of South African estuarine microbial species and genome diversity
(University of the Western Cape, 2006)A study of the microbial diversity in sediments of the Great Berg River estuary is carried out using modern molecular phylogenetic methods. The aim of this study was to determine the effect of (pollution by) the effluents ... -
Iterative helical real-space reconstruction of histone octamer tubular crystals and implications for the 30 nm chromatin fiber
(University of the Western Cape, 2006)This thesis investigated the helical structure of core histone octamers to discover interacting surfaces and their relevance to the compaction of nucleosome arrays into the chromating fiber. -
Screening of natural products and alkylating agents for antineoplastic activity
(2007)Apoptosis is a process in which a cell programmes its own death. It is a highly organized physiological mechanism in which injured or damaged cells are destroyed. Apart from physiological stimuli however, exogenous factors ... -
An investigation of the zinc binding characteristics of the RING finger domain from the human RBBP6 protein using heteronuclear NMR spectroscopy.
(University of the Western Cape, 2007)Retinoblastoma binding prot ein 6 (RBBP6) is a 250 kDa human splicing-associated protein that is also known to interact with tumour suppresso r proteins p53 and pRb and to mediate ubiquitination of p53 via its ... -
Genetic analysis of resistance to apple scab (Venturia inaequalis) in apple (Malus x domestica Borkh)
(University of the Western Cape, 2007)Amongst the many problems facing the apple industry, apple scab is one of the most challenging experienced by producers. This disease is caused by Venturia inaequalis, which causes lesions to develop on both the fruit and ... -
Screening of natural products and alkylating agents for antineoplastic activity
(University of the Western Cape, 2007)Background and objectives: Apoptosis is a process in which a cell programmes its own death. It is a highly organized physiological mechanism in which injured or damaged cells are destroyed. Apart from physiological stimuli ... -
Spartial distribution of cyanobacterial phylotypes in Antarctic Dry Valley soil biotopes
(University of the Western Cape, 2007)Recent advances in moleculr methods have enabled the analysis of cyanobacterial diversity using PCR- based approaches. Snap-shot techniques such as 16S rDNA library construction and DGGE have allowed for increased access ... -
Engineering homoaromatic substrate specificity into aliphatic-specific Geobacillus pallidus RAPc8 nitrile hydratase
(University of the Western Cape, 2007)Geobacillus pallidus RAPc8 is a thermophilic nitrile-degrading isolate, obtained from thermal sediment samples of a New Zealand hot spring. The G. pallidus RAPc8 NHase gene has been cloned and expressed in E. coli. The ... -
Analysis of the proteome of red and green leaf phenotypes of 'Bon Rouge' pear trees \trus communrls L. by 2-dimensional gel electrophoresis
(University of the Western Cape, 2007)The 'Bon Rouge' pear is a red pear cultivar derived from a rare, spontaneous bud mutation which occurred on the green pear 'William's Bon Chretien' (Bartlett). 'Bon Rouge' pear cultivar was observed to be reverting back ... -
identification of Quantitative Tralt Locl Controlling the Requirement for chilling ln Vegetative budbreak in apple (malus x domestica Borkh')
(University of the Western Cape, 2007)The domesticated apple (Malus x domestica Borkh.) has been distributed into diverse climatic conditions worldwide for commercial production of fruit. Apple trees need exposure to cold temperatures, referred to as chill ... -
Identification of quantitative trait loci control l ing the requirement for chilling in vegetative budbreak in apple (malus x domestica borkh.)
(2008)The domesticated apple (Malus x domestica Borkh.) has been distributed into diverse climatic conditions worldwide for commercial production of fruit. Apple trees need exposure to cold temperatures, referred to as chill ...