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The structure of the nitrilase from Rhodococcus Rhodochrous J1: homology modeling and three-dimensional reconstruction
(University of the Western Cape, 2006)
The nitrilases are an important class of industrial enzymes that are found in all phyla. These enzymes are expressed widely in prokaryotes and eukaryotes. Nitrilases convert nitriles to corresponding acids and ammonia. ...
Recombinant expression of the pRb- and p53-interacting domains from the human RBBP6 protein for in vitro binding studies
(University of the Western Cape, 2004)
This thesis describes the cloning and recombinant expression of domains from the human RBBP6 protein for future in vitro binding studies with pRb and p53. RBBP6 is a splicing-associated protein that is known to interact ...
Comparison of multi-gene integration strategies in CRISPR-based transformation of Saccharomyces cerevisiae
(University of the Western Cape, 2021)
Saccharomyces cerevisiae is an important host in industrial biotechnology. This yeast is the host of choice for the first and second-generation biofuels for ethanol production. Genome modification in S. cerevisiae has been ...
Biocontrol potential of fungal endophytes against Fusarium proliferatum in maize
(University of the Western Cape, 2022)
Fusarium proliferatum is a fungal pathogen that is the cause of numerous diseases in various
crops of fruit and vegetables. About 25% of the maize crops harvested annually are affected by
mycotoxins produced by F. ...
The effect of chitosan in the germination and growth of sorghum bicolor under salt stress
(University of the Western Cape, 2022)
The agricultural sector has been facing enormous challenges including reduced crop yield and increased food production demand to cater for the growing population. Crop production is severely affected by abiotic and biotic ...
Exogenous carbon monoxide and jasmonic acid mitigate salt stress in Sorghum bicolor
(University of the Western Cape, 2022)
The agricultural sector plays an important role in the world economy. Expanding research is
piloting its improved contribution to the economic sector. Despite the economic gain, the
increasing population has already put ...
Using CRISPR/Cas9 to construct consolidated bioprocessing strains from natural isolates of Saccharomyces cerevisiae
(University of the Western Cape, 2022)
Saccharomyces cerevisiae has gained much attention as a host for cellulosic bioethanol production using consolidated bioprocessing (CBP) methodologies, due to its high ethanol producing titres, heterologous protein producing ...
Investigation of the anti-obesity effects of citrate-capped gold nanoparticles on 3T3-L1 cells and their genetic profiling
(University of the Western Cape, 2023)
The incidence and prevalence of obesity and its related diseases has nearly tripled and is increasing at an alarming rate worldwide. Several modern methods for the management and treatment of obesity are available, with ...