Browsing Department of Biotechnology by Title
Now showing items 102-121 of 362
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A dual analysis of the South African Griqua population using ancestry informative mitochondrial DNA and discriminatory short tandem repeats on the Y chromosome
(University of the Western Cape, 2015)The primary objective of this Masters project was to investigate the maternal ancient substructure of the Griqua population in South Africa. Genetic ancestry was determined by investigating ancestry informative single ... -
The effect of blocking selected endocytic mechanisms on heterologous protein secretion in the yeast saccharomyces cerevisiae
(University of the Western Cape, 2018)The yeast Saccharomyces cerevisiae is considered a good host used for heterologous protein production due to the organism’s microbial safety, rapid growth and eukaryotic post- translational processing. As a fermentative ... -
The effect of chitosan in the germination and growth of sorghum bicolor under salt stress
(University of the Western Cape, 2022)The agricultural sector has been facing enormous challenges including reduced crop yield and increased food production demand to cater for the growing population. Crop production is severely affected by abiotic and biotic ... -
Effect of Fusarium oxysporum on the physiological and molecular responses of cowpea plants
(The University of the Western Cape, 2017)Cowpea is a tropical, drought-tolerant legume crop that is indigenous to Africa. The crop is of nutritional importance to both animals and humans as part of their diet. Stress to crop plants is defined as any factor that ... -
Effects of exogenous myristic acid on growth and germination of Brassica napus L. under zirconium toxicity
(University of the Western Cape, 2016)Lipids when exogenously applied are known to cause various changes in ROS levels produced within plants. They can either be beneficial to the plant when not stimulating the overproduction of ROS thus resulting in improved ... -
The effects of gallic acid on the membrane proteome and antioxidant system of wheat plants under salt stress
(University of Western Cape, 2020)Salt stress is a major abiotic stress that accounts for huge agricultural losses worldwide, which in turn threaten food security and sustainable agriculture. Salt triggers the excessive production of reactive oxygen species ... -
Effects of nitric oxide and hydrogen peroxide on antioxidant enzyme activity in zea mays subjected to drought
(University of Western Cape, 2021)Agricultural practices are significantly affected by drought. Drought is one of the most important plant stresses, causing several physiological, morphological, biochemical, and molecular changes in plants. Drought stress ... -
Effects of nitric oxide on novel soybean cystatin gene expression under salt stress in soybean
(University of the Western Cape, 2012)Nitric oxide (NO) has been shown to orchestrate multiple defense responses to both abiotic and biotic stress. Importantly, elevation of nitric oxide content in plants by using nitric oxide generating compounds has been ... -
The effects of nitric oxide on soybean superoxide dismutase activity during osmotic stress
(University of the Western Cape, 2012)Nitric oxide (NO) is a signaling molecule involved in mediating plant responses to various biotic and abiotic stresses. Major abiotic stresses (drought, salinity, cold) induce common cellular responses, causing osmotic ... -
Electroanalysis of Organo-Chalcogenic Perovskite Nanomaterials
(University of the Western Cape, 2017)Organometallic halide perovskite solar cells have developed as one of the most promising contenders for the production of solar cells. The generic perovskite (PS) structure ABX3 allows manufacturing a broad range of PS ... -
Enabling the production of black soldier fly larval protein from lignocellulosic biomass through pre-treatment and enzymatic hydrolyse
(University of the Western Cape, 2023)Due to the rapid increase in the world’s population, alternative food resources are crucial to sustain the human diet. Insects can produce valuable proteins and nutrients that can be used as high-quality animal feedstuff ... -
Engineering bacterial magnetic nanoparticles
(University of the Western Cape, 2013)Magnetosomes, produced by magnetotactic bacteria (MTB), are the most attractive alternative source of non-toxic biocompatible magnetic nanoparticles (MNPs). A magnetosome contains Fe2O4 magnetite with properties superior ... -
Engineering homoaromatic substrate specificity into aliphatic-specific Geobacillus pallidus RAPc8 nitrile hydratase
(University of the Western Cape, 2007)Geobacillus pallidus RAPc8 is a thermophilic nitrile-degrading isolate, obtained from thermal sediment samples of a New Zealand hot spring. The G. pallidus RAPc8 NHase gene has been cloned and expressed in E. coli. The ... -
Engineering Parageobacillus thermoglucosidans as a robust platform for bioethanol production
(University of the Western Cape, 2018)Parageobacillus thermoglucosidans is a promising “platform” organism to use in the production ofa range of useful metabolites with demonstrated ability to produce ethanol, isobutanol and polylactic acid for bio-degradable ... -
Eukaryotic diversity of miers valley hypoliths
(University of the Western Cape, 2012)The extreme conditions of Antarctic desert soils render this environment selective towards a diverse range of psychrotrophic microbial communities. Cracks and fissures in translucent quartz rocks permit an adequate amount ... -
Evaluating the specancer cell targeting peptides for applications in cancer diagnostics
(University of the Western Cape, 2013)Cancer is a disease most often associated with poor prognosis. During the development of the disease, cells acquire genetic mutations which result in changes in bio-molecules (DNA and protein), thus altering normal ... -
Evaluation of agronomic and drought response traits in two cultivars of soybean for cultivation in the Transkei region of the Eastern Cape
(University of Western Cape, 2020)Drought is a major stress that affects the productivity of plants across the globe. The consequences of drought are posed to continue reducing agricultural productivity as world climate changes. This makes it imperative ... -
Evaluation of cytotoxic activity of gold nanoparticles naturally synthesised from South African indigenous medicinal plant extracts
(University of the Western Cape, 2018)Nanotechnology has emerged as a promising field in the quest to address health conditions. Green nanotechnology is a fairly new branch of nanotechnology, which aims to produce and utilize nanomaterials in a way that is ... -
Evaluation of insertion-deletion polymorphisms with the kit Qiagen Investigator® DIPplex for forensic application in South Africa
(University of the Western Cape, 2015)Insertion-deletion polymorphisms (indels) have been underutilized in forensic identification of individuals in comparison with single nucleotide polymorphisms (SNPs) and short tandem repeat (STRs) systems. The use of ... -
Evaluation of the anticancer and molecular effects of green synthesized silver nanoparticles
(University of the Western Cape, 2023)Globally, cancer is considered the second leading cause of death with a staggering tally of 10 million deaths. Breast, lung, colon, rectum and prostate cancers are amongst the most commonly diagnosed cancers. The current ...