Browsing Department of Biotechnology by Title
Now showing items 193-212 of 362
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The in vitro detection and measurement of the unfolded protein response in Saccharomyces cerevisiae
(University of the Western Cape, 2018)Bioethanol is currently the most widely used biofuel and can be used as a direct replacement for current fossil fuel based transportation fuels. Consolidated bioprocessing (CBP), in which bioethanol is produced in a single ... -
In vitro evaluation of Leonotis leonurus to validate its potential as an alternative anti-diabetic drug
(University of the Western Cape, 2022)Africa is a rich source of medicinal plants with South Africa being known as a country with a strong history of traditional healing, which masses a variety of approximately 30 000 flowering plants and accounting for ... -
In vitro investigation of putative interactions between the RING finger domain of Retinoblastoma Binding Protein 6 (RBBP6) and various substrates
(University of the Western Cape, 2015)Retinoblastoma Binding Protein 6 (RBBP6) is a RING finger-containing protein which plays a critical role in the 3'-end processing of mRNA transcripts. It is a constituent of the human pre-mRNA processing complex but also ... -
In vitro investigation of the ubiquitination and degradation of p53 by Murine Double Minute 2 (MDM2) and Retinoblastoma Binding Protein 6 (RBBP6)
(University of the Western Cape, 2015)P53 is one of the most important tumour suppressor proteins in the body which protects the cell against the tumourigenic effects of DNA damage by initiating processes such as apoptosis, senescence and cell cycle arrest. ... -
In-silico optimization and molecular validation of putative anti-HIV antimicrobial peptides for therapeutic purpose
(University of the Western cape, 2016)AIDS is considered a pandemic causing millions of deaths worldwide and a cure for this disease is still not available. Failure to implement early treatments due to the poor diagnostic methods and ineffective therapeutic ... -
Increasing ethanol tolerance through the heterologous expression of the geobacillus thermoglucosiadius heat-shock proteins (GroEL/GroES) in an escherichia coli host
(University of the Western Cape, 2012)Due to economic and environmental concerns associated with use of fossil fuels, humanity is seeking alternative fuels. Ethanol is one of the alternative fuels produced commercially. Current ethanol production technologies ... -
Influence of a selected endophyte consortium on salinity responses in Medicago sativa
(University of Western Cape, 2022)Salinity is one of the major limiting factors to crop production, which consequently contributes to the risk of reduced food security. Among other factors, food security depends on availability of sufficient and nutritious ... -
Inter-individual genetic variation and the development of hypertension in a Xhosa African population of Eastern Cape, South Africa
(University of the Western Cape, 2022)Cardiovascular diseases (CVD) are the leading cause of death globally, accounting for 18.6 million deaths. Hypertension (HTN) drives the global burden of CVD and is a leading cause of cardiovascular-related mortality with ... -
The interactive effects of salt stress and Fusarium proliferatum infection on maize seedlings
(University of the Western Cape, 2022)Field crops are often subjected to multiple co-occurring stress factors, and they evolved specific mechanisms to counteract the effects of these stress factors. Most studies explore the fundamental molecular mechanisms ... -
The Internal Validation and Casework Application of MiniSTR Systems
(University of the Western Cape, 2008)The objective of the study was to conduct an internal validation on miniSTR systems and apply it to cases received from the South African Missing Persons Task Team (SAMPTT). This was prompted by the fact that miniSTR systems ... -
Investigating the antimicrobial potential of Thalassomonas actiniarum
(University of the Western Cape, 2020)bioassay guided isolation approach was then used to isolate the high molecular weight antibacterial compound (50kDa-100kDa) from T. actiniarum fermentations. With common protein isolation, purification and detection methods ... -
Investigating the antimicrobial potential of Thalassomonas actiniarum
(University of Western Cape, 2020)The World Health Organisation predicts that by the year 2050, 10 million people could die annually as a result of infections caused by multidrug resistant bacteria. Individuals with compromised immune systems, caused by ... -
Investigating the effect of particle size on the antibacterial activity of gold nanoparticles
(University of the Western Cape, 2017)The increase of antibiotic- and/or multidrug-resistant bacteria has become a major global challenge. Killing of antibiotic-resistant bacteria requires a high dose of multiple, expensive drugs, which possess unfavourable ... -
Investigating the effect of particle size on the antibacterial activity of gold nanoparticles
(University of the Western Cape, 2017)The increase of antibiotic- and/or multidrug-resistant bacteria has become a major global challenge. Killing of antibiotic-resistant bacteria requires a high dose of multiple, expensive drugs, which possess unfavourable ... -
Investigating the functional interaction of transcription regulator CarD of Mycobacterium tuberculosis with Ribonucleic Acid Polymerase
(University of the Western Cape, 2014)Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (Mtb). TB mainly affects lungs of patients but other parts of the body can also be affected. It kills approximately 2 million people annually. ... -
Investigating the functional interaction of transcription regulator card of mycobacterium tuberculosis with ribonucleic acid polymerase
(2013)Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (Mtb). TB mainly affects lungs of patients but other parts of the body can also be affected. It kills approximately 2 million people annually. ... -
An investigation into the antimicrobial and immune modulatory effects of Sutherlandia frutescens and Salvia africana-lutea extracts and their respective biogenic nanoparticles
(University of the Western Cape, 2020)The prevalence of cancer and antibiotic-resistant microbes has led to the necessity of affordable, efficacious, non-toxic and easily accessible therapeutic approaches. There is numerous anecdotal evidence that African ... -
Investigation into the role of DWNN in cell death
(University of the Western Cape, 2004)Many genes are activated to influence the self-destruction programme of the cell. This programme entails synchronised instigation and implementation of numerous subprograms. The arrival of gene targeting aided in the ... -
Investigation of Guayule's Agronomic Performance and Agro-processing in South Africa
(University of the Western Cape, 2017)Guayule (Parthenium argentatum) is grown for its high quality hypoallergenic natural rubber latex production. The plant is native to the Chihuahua desert of North America and successful trials have been done in Europe, the ... -
Investigation of protein-metal ion and protein-protein interactions using mass spectrometry and nuclear magnetic spectroscopy
(University of Western Cape, 2014)Protein-protein interaction networks provide a global picture of cellular function and biological processes. Some proteins act as hub proteins, highly connected to others, whereas some others have few interactions. The ...