Browsing Department of Biotechnology by Title
Now showing items 235-254 of 362
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Isolation and characterization of stem endophytic bacteria from weed plants for enhancing Vanadium tolerance in Brassica napus
(University of the Western Cape, 2019)Bacterial endophytes are able to improve the growth of their hosts through a number of different mechanisms such as nutrient uptake regulation, plant hormone production and regulation, siderophore production and phosphate ... -
Isolation and identification of PNP-A homologues from Xerophyta viscosa Baker
(University of the Western Cape, 2004)Maintenance of growth, development and normal physiological processes remains crucial for the survival of plants. Plant hormones are described as integrators of external signal and internal developmental process in plants. ... -
Isolation and preliminary characterization of bacteriophages of thermophilic Bacillus and Geobacillus species
(University of the Western Cape, 2015)Thermophilic bacteriophages provide simple model systems for understanding biochemical and biological adaptation mechanisms at elevated temperatures. The essential objectives of this study were to characterise the ... -
Isolation, expression, purification and characterisation of a novel acetyl xylan esterase from streptomyces species ORS10
(University of the Western Cape, 2012)Lignocellulosic biomass represents an important renewable resource for biofuels production. Lignocellulosic biomass is comprised of cellulose, hemicellulose and lignin. Lignocellulosics are highly recalcitrant to enzymatic ... -
Isolation, identification and characterisation of novel actinobacteria from Zambian hot-springs
(2011)Actinomycetes are ubiquitous in many environments such as soil, activated sludge and water.Besides the genus Streptomyces, which has been extensively exploited, members of other genera including Micromonospora have been ... -
Isolation, identification and characterization of novel actinomycetes from Antarctic soil samples
(University of the Western Cape, 2008)Actinomycetes are Gram-positive bacteria that form filamentous branching hyphae with asexual spores. They occur in a variety of environments and are important industrially and pharmaceutically. The aims of this study were ... -
Iterative helical real-space reconstruction of histone octamer tubular crystals and implications for the 30 nm chromatin fiber
(University of the Western Cape, 2006)This thesis investigated the helical structure of core histone octamers to discover interacting surfaces and their relevance to the compaction of nucleosome arrays into the chromating fiber. -
Laccases from actinomycetes for lignocellulose degradation
(University of the Western Cape, 2012)Lignocellulose has a complex structure composed mainly of lignin, hemicellulose and cellulose. Several enzymes are needed for the degradation of lignocellulose into simple sugars. Actinomycetes are known to produce laecases ... -
Laccases from actinomycetes for lignocellulose degradation
(University of the Western Cape, 2012)The purpose of this study shows that lignocellulose has a complex structure composed mainly of lignin, hemicellulose and cellulose. Several enzymes are needed for the degradation of lignocellulose into simple sugars. ... -
Manipulating biotic and abiotic factors to enhance the remediation of agri-industrial wastewater in pilot-scale constructed wetlands
(University of the Western Cape, 2012)As a consequence of various cellar activities the wine industry produces copious volumes of potentially hazardous wastewater each year. South Africa is one of the top ten wine-producing countries, making the successful ... -
Mapping and identification of disease resistance candidate genes in three Malus populations using SSRs, DArT and Infinium SNP markers and Illumina sequencing technology
(University of the Western Cape, 2014)Apple scab, powdery mildew and woolly apple aphid are a major concern for apple breeders and producers. Control of these diseases is a significant economic and marketing priority for the South African apple industry. ... -
Marine bacteria as a potential source for novel antimicrobial compounds
(University of the Western Cape, 2020)The high rate of rediscovery of known compounds has led to a decline in the discovery of novel natural products. The high biodiversity of organisms growing in extreme conditions such as oceans has led to the increased ... -
Massively-Parallel Computational Identification of Novel Broad Spectrum Antivirals to Combat Coronavirus Infection
(University of the Western Cape, 2015)Given the significant disease burden caused by human coronaviruses, the discovery of an effective antiviral strategy is paramount, however there is still no effective therapy to combat infection. This thesis details the ... -
Matrix comparison of isolation conditions for secondary metabolite producing marine sponge associated bacteria
(University of the Western Cape, 2015)The discovery of novel secondary metabolites has declined significantly in recent years whereas there is a rise in the number of multi-drug resistant pathogens and other types of diseases. The decline in natural product ... -
Metagenomic approaches to gene discovery
(University of the Western Cape, 2006)The classical approach to gene discovery has been to culture micro-organisms demonstrating a specific enzyme activity and then to recover the gene of interest through shotgun cloning. The realization that these standard ... -
Methylgyoxal signalling in Phaseolus vulgaris under phosphate deficiency
(UWC, 2020)In this study, we observed that phosphate (P) deficiency stunted plant growth and produced plants with poor morphological characteristics (yellow and small leaves). Furthermore, we treated plants with 0.8 mM (control) and ... -
The microbial composition of a natural methanogenic consortium
(University of the Western Cape, 2005)Wetlands account for approximately 20% of annual global methane emissions. Many wetlands receive inputs of organic matter, nutrients, metals and various toxic compounds from adjacent agricultural and industrial areas. The ... -
Microbial diversity and gene mining in Antarctic Dry Valley mineral soils
(University of the Western Cape, 2006)Soil communities are regarded as among the most complex and diverse assemblages of microorganisms with estimated bacterial numbers in the order of 109-1 cells.g. Studies on extreme soils however, have reported lower cell ... -
Microbial diversity of Antarctic Dry Valley mineral soil
(University of the Western Cape, 2004)Antarctica provides some of the most extreme environments on earth. Low temperatures, low water availability and nutrient deficiency are contributing factors to the limited colonisation of Antarctic biotopes, particularly ... -
Microbial diversity of the Namib Desert salt pans
(University of the Western Cape, 2015)Salt pans are a characteristic feature of many dry deserts. The microbial communities inhabiting salt pans are thought to be particularly complex and are generally dominated by halophilic microorganisms. Although saline ...