Browsing Department of Biotechnology by Title
Now showing items 295-314 of 361
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Proteomic and functional characterisation of biofilm formation in Xylophilus ampelinus
(University of the Western Cape, 2019)Xylophilus ampelinus, the causal agent of bacterial blight of grapevine, inhabits the vascular tissues of infected plants where the cells appear to form assemblages resembling biofilms. Bacterial blight of grapevine ... -
Proteomic and metabolomic characterisation of novel wine yeasts: towards the evaluation and improvements of their ability to produce aromatic sauvignon blanc wines
(University of the Western Cape, 2017)Wine yeast Saccharomyces cerevisiae and wild yeasts e.g. Torulaspora delbrueckii forms an integral part of wine production by converting relatively 'neutral' flavoured Sauvignon blanc grape must into varietal aromatic ... -
Proteomic and SNP analysis of the Cadherin 10 type-II (CDH10) gene, in the South African autistic population
(University of the Western Cape, 2013)Autism or autism spectrum disorder (ASD) is a very diverse neurological disorder that manifests specifically in children and infants between the ages of two to three years of age. An individual suffering is deemed as ... -
Proteomic characterisation of wine yeast strains for the expression of arginases involved in urea formation during fermentation
(University of the Western Cape, 2018)Wine is a fermented beverage widely consumed all over the world as a recreational drink, but is known for its health benefits to humans. However, wine contain urea, a by-product of arginine hydrolysis by arginases expressed ... -
Public participation as governance the role of catchment forums in water governance Mluleki Matiwane
(2012)Catchment forums are concerned groups of people that come together on a voluntary basis in a specific area. They share the same goal and purpose to achieve - sustainable water resources management. These Catchment Forums ... -
Recombinant expression and full backbone assignment of the human DWNN using heteronuclear NMR
(University of the Western Cape, 2005)The cellular levels of a number of proteins have been found to be regulated by the ubiquitin-proteasome pathway. In this pathway, proteins are covalently tagged (“ubiquitinated”) by ubiquitin, which acts as a signal for ... -
Recombinant expression of cytochrome P450-2D6 and its application in tamoxifen metabolism
(University of the Western Cape, 2018)Breast cancer is regarded as the most common form of cancer in women and it comprises of approximately 23 % of female cancers, while affecting women at any age range. For oestrogen receptor positive patients, tamoxifen is ... -
Recombinant expression of the pRb- and p53-interacting domains from the human RBBP6 protein for in vitro binding studies
(University of the Western Cape, 2004)This thesis describes the cloning and recombinant expression of domains from the human RBBP6 protein for future in vitro binding studies with pRb and p53. RBBP6 is a splicing-associated protein that is known to interact ... -
Recombinant expression of the pRb- and p53-interacting domains from the human RBBP6 protein for in vitro binding studies
(University of the Western Cape, 2004)This thesis describes the cloning and recombinant expression of domains from the human RBBP6 protein for future in vitro binding studies with pRb and p53. RBBP6 is a splicing-associated protein that is known to interact ... -
Reconstruction of major male and female lineages of the kensington Muslim community
(University of the Western Cape, 2010)The earliest Cape Muslims were brought to the Cape from Africa and Asia from 1652 to 1834. They were part of an involuntary migration of slaves, political prisoners and convicts, and they contributed in the ethnic diversity ... -
Reconstruction of major male and female lineages of the Strand Muslim community
(University of the Western Cape, 2010)Initially, a pilot study was carried out in order to reconstruct the major paternal and maternal lineages of the Muslim population living in the Cape metropolitan area. The Study has shown the ability of molecular genetic ... -
The relationship between structure and thermostability of a nitrile hydratase from Goebacillus pallidus RAPc8
(University of the Western Cape, 2008)Nitrile hydratases (NHases) are very important biocatalysts for the enzymatic conversion of nitriles to industrially important amides such as acrylamide and nicotinamide. An “ideal” NHase should fulfil several essential ... -
Respiratory and photosynthetic C and N metabolism of nodulated Lupin roots during phosphorus deficiency
(University of the Western Cape, 2010)Growth of symbiotic legume hosts is P limited, because of the high energetic requirements associated with N2 fixation. Attempts to overcome P deficiency in soils where legumes are grown involve addition of P-based fertilisers. ... -
Response of microbial communities to synthetic winery wastewater in biological sand filters
(University of the Western Cape, 2017)There has been a significant increase in the use of constructed wetlands globally for the bioremediation of wastewater (Welz et al., 2011). In South Africa, the wine industry generates more than one billion litres of ... -
Responses of maize Antioxidant Enzymes to drought stress
(University of the Western Cape, 2019)Maize (Zea mays L) was subjected to drought stress for 28 days. The effects of the drought stress on growth, H2O2 content and lipid peroxidation were investigated and the activities of antioxidant enzymes [superoxide ... -
Responses of maize antioxidant enzymes to drought stress
(University of the Western Cape, 2019)Maize (Zea mays L) was subjected to drought stress for 28 days. The effects of the drought stress on growth, H2O2 content and lipid peroxidation were investigated and the activities of antioxidant enzymes [superoxide ... -
The role of p-coumaric acid on physiological and biochemical response of chia seedling under salt stress
(University of the Western Cape, 2020)The role of phenolic acids in mitigating salt stress tolerance have been well documented. However, there are contradicting reports on the effect of exogenously applied phenolic acids on the growth and development of various ... -
Sample preparation methods and molecular based detection for the rapid isolation and identification of Listeria monocytogenes in food samples
(University of the Western Cape, 2006)Listeria monocytogenes is a Gram-positive bacterium responsible for listeriosis, a food-borne disease, which may result in severe illness and possible death. The importance of L. monocytogenes as a food-borne pathogen has ... -
Saturation sequencing, characterisation and mapping of the NBS-LRR resistance gene family in apple, Malus x domestica (Borkh)
(University of the Western Cape, 2008)To date five classes of resistance proteins have been identified in plants and these include the intracellular protein kinases, receptor-like protein kinases with extracellular leucinerich repeat (LRR) domain, LRR proteins ... -
Screening bacterial symbionts of marine invertebrates for ribosomally synthesized natural products
(University of the Western Cape, 2016)Pharmaceutical research and development strategies rely on the constant discovery of novel natural products as potential drugs. Recent studies have shown that the microorganisms associated with sponges are the true producers ...