Browsing Department of Biotechnology by Title
Now showing items 62-81 of 362
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Comparison of actinobacterial diversity in Marion Island terrestrial habitats
(University of the Western Cape, 2008)The major aim of this study is to determine and compare the distribution of bacteria and actinobacteria in Marion Island terrestrial habitats. -
Comparison of multi-gene integration strategies in CRISPR-based transformation of Saccharomyces cerevisiae
(University of Western Cape, 2021)Saccharomyces cerevisiae is an important host in industrial biotechnology. This yeast is the host of choice for the first and second-generation biofuels for ethanol production. Genome modification in S. cerevisiae has been ... -
Comparison of multi-gene integration strategies in CRISPR-based transformation of Saccharomyces cerevisiae
(University of the Western Cape, 2021)Saccharomyces cerevisiae is an important host in industrial biotechnology. This yeast is the host of choice for the first and second-generation biofuels for ethanol production. Genome modification in S. cerevisiae has been ... -
Comparison of plasmids from clinical Lactobacillus strains
(University of the Western Cape, 2018)The vaginal mucosa is dominated by Gram positive, rod shaped lactobacilli which serve as a natural barrier against infection. In both healthy and BV infected women Lactobacillus crispatus and Lactobacillus jensennii has ... -
Comparison of plasmids from clinical Lactobacillus strains
(University of the Western Cape, 2018)The vaginal mucosa is dominated by Gram positive, rod shaped lactobacilli which serve as a natural barrier against infection. In both healthy and BV infected women Lactobacillus crispatus and Lactobacillus jensennii has ... -
A computational framework for transcriptome assembly and annotation in non-model organisms: the case of venturia inaequalis
(University of the Western Cape, 2014)In this dissertation three computational approaches are presented that enable optimization of reference-free transcriptome reconstruction. The first addresses the selection of bona fide reconstructed transcribed fragments ... -
Deciphering a potential cytoprotective role of novel heat shock responsive proteins using a proteomic approach
(University of the Western Cape, 2011)Myocardial infarction, commonly known as a heart attack, is a condition where the blood supply to the heart tissue is cut off, starving the tissue from oxygen and nutrient supply, with consequent lethal damage to the heart ... -
Deciphering the determinants of molecular physiological responses to drought and heat stress in sorghum lines contrasting in stress tolerance via a proteomics approach
(University of the Western Cape, 2022)Sorghum is an important crop in Sub-Saharan Africa and South Asia. The predicted rise in global temperatures will increase the probability of exposing sorghum to heat waves in combination with drought. Thus, production ... -
Defining the African green monkey (Chlorocebus Aethiops): expression behaviour of selected lipid metabolism genes in response to niacin
(University of Western Cape, 2012)In this century most major medical advances have resulted in part from research on animals and non-human primates such as the African green monkey and therefore often serve as a critical link between basic research and ... -
The degradation of the endocrine disrupting chemical, bisphenol-A : a comparative study between fungal and bacterial laccases
(University of the Western Cape, 2015)The degradation of endocrine disrupting chemicals (EDCs) is a topic of high importance and one that research efforts are continually being focused on. These harmful chemicals are known to cause adverse health effects in ... -
The detection of meningococcal disease through identification of antimicrobial peptides using an in silico model creation
(University of the Western Cape, 2019)Neisseria meningitidis (the meningococcus), the causative agent of meningococcal disease (MD) was identified in 1887 and despite effective antibiotics and partially effective vaccines, Neisseria meningitidis (N. meningitidis) ... -
Developing a generic hazard analysis critical control point (HACCP) system for the wheat milling industry
(University of the Western Cape, 2005)In South Africa there is a need within the milling industry for controlling food safety especially due to customer's demands and government's regulations. The best way to ensure food safety is with the implementation of a ... -
Developing a lateral flow device for the detection of novel breast cancer biomarkers
(University of the Western Cape, 2023)Breast cancer is the most prevalent malignancy and one of the leading causes of death in women worldwide. Delayed diagnosis of breast cancer greatly reduces the effectiveness of treatment and is considered one of the major ... -
Developing a quantum dot nanotracker for endophytic bacteria translocation in plants
(University of the Western Cape, 2017)Endophytes are bacteria that have been shown in previous studies to promote plant growth without eliciting any sign of infection or plant disease, as well as promote bio-fortification and has thus gained much attention ... -
Development and validation of a pharmacogenomics profiling panel suitable for personalizing metformin therapy
(University of the Western Cape, 2019)The burden of non-communicable diseases (NCDs) in South Africa is predicted to increase substantially in the next decades if the necessary preventative measures are not taken. The two most common NCDs associated with ... -
Development and validation of a pharmacogenomics profiling panel suitable for personalizing Metformin therapy
(University of the Western Cape, 2019)The burden of non-communicable diseases (NCDs) in South Africa is predicted to increase substantially in the next decades if the necessary preventative measures are not taken. The two most common NCDs associated with rapid ... -
Development and validation of Non-CODIS miniSTR genotyping systems suitable for forensic case work in South Africa
(University of the Western Cape, 2010)The objective of this study was to develop and validate a six Non-CODIS miniSTR genotyping system and to determine its suitability for forensic casework in South Africa. In Non-CODIS miniSTR genotyping systems, smaller PCR ... -
Development of a COMT PCR multiplex to investigate resilience, anxiety and childhood trauma in a South African population
(University of the Western Cape, 2020)Anxiety, resilience and childhood trauma can be categorized as functional behavioural categories, with a wealth of research behind each. The research approach adopted for each, in most cases, is either from a genetic or ... -
Development of a high throughput cell-free metagenomic screening platform
(University of the Western Cape, 2016)The estimated 5 × 10³⁰ prokaryotic cells inhabiting our planet sequester some 350–550 Petagrams (1 Pg = 1015 g) of carbon, 85–130 Pg of nitrogen, and 9–14 Pg of phosphorous, making them the largest reservoir of those ... -
Development of a novel high throughput method for identifying phage-host pairs in an extreme environment
(University of the Western Cape, 2017)There are approximately 10³¹ bacteriophages in the biosphere, outnumbering bacteria 10:1, hence, the dynamic and specific nature of phage-host interactions exerts significant influence on microbial communities. Bacteriophages ...