Browsing Philosophiae Doctor - PhD (Biotechnology) by Issue Date
Now showing items 21-40 of 98
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A proteomic analysis of drought and salt stress responsive proteins of different sorghum varieties
(University of the Western Cape, 2009)This study reports on a proteomic analysis of sorghum proteomes in response to salt and hyperosmotic stresses. Two-dimensional gel electrophoresis (2DE) in combination with mass spectrometry (MS) was used to separate, ... -
Characterisation of AtPNP-A - a novel arabidopsis thaliana gene with role in water and salt homeostasis
(University of the Western Cape, 2009)Plant natriuretic peptides (PNPs) are a novel class of extracellular, systemically mobile molecules that elicit a number of plant responses important in homeostasis and growth. Natriuretic peptides were first identified ... -
Plant natriuretic peptides - elucidation of the mechanisms of action
(University of the Western Cape, 2009)Several lines of cellular and physiological evidence have suggested the presence of a novel class of systemically mobile plant molecules that are recognized by antibodies generated against vertebrate atrial natriuretic ... -
Identification of bacterial pathogenic gene classes subject to diversifying selection
(University of the Western Cape, 2009)Availability of genome sequences for numerous bacterial species comprising of different bacterial strains allows elucidation of species and strain specific adaptations that facilitate their survival in widely fluctuating ... -
Functional genomic characterization of fruit quality traits in apple (Malus x domestica Borkh)
(University of the Western Cape, 2009)The domesticated apple (Malus x domestica Borkh), belonging to the Malusgenus of the Rosaceae family, is one of the edible pomaceous fruits. Since it is one of the important commercial fruit crops worldwide, the quality ... -
Molecular detection and characterisation of RNA viruses of honeybees
(University of the Western Cape, 2009)Honeybees have evolved through the centuries to inhabit most parts of the world except for the extreme Polar Regions. These insects have also been susceptible to pathogens and disease which has always been part of the ... -
Genome-wide identification and comprehensive analysis of transcriptionsl desert regions
(University of the Western Cape, 2009)The initiation of transcription in mammalian genomes predominatly occurs at 5' promoter regions, however increasingly initiation events have been observed within introns, coding exons and 3' UTRs. Nevertheless there ... -
A proteomic analysis of drought and salt stress responsive proteins of different sorghum varieties
(University of the Western Cape, 2009)Sorghum (Sorghum bicolorï, a drought tolerant cereal crop, is not only an important food source in the semi arid/arid regions but also a potential model for studying and gaining a better understanding of the molecular ... -
A molecular investigation of the prevalence of suspected periodontopathogens and their association with preterm birth
(University of the Western Cape, 2010)More than 20 million infants in the world (15.5 % of all births) are born with low birth weight. Ninety-five % of them are in developing countries. Oral colonization of Gramnegative anaerobes has been implicated as a risk ... -
Molecular Characterisation of the Brassinosteroid, Phytosulfokine and cGMP-dependent Responses in Arabidopsis thaliana
(University of the Western Cape, 2010)In this thesis, we have firstly cloned and expressed the domains that harbours the putative catalytic GC domain in these receptor molecules and demonstrate that these molecules can convert GTP to cGMP in vitro. Secondly, ... -
Structural characterisation of the interaction between RBBP6 and the multifunctional protein YB-1
(University of the Western Cape, 2010)As a means of further localising the interaction, truncated fragments derived from the C-terminal region of YB-1, were tested for their interaction with the RING finger domain of RBBP6 using three different assays: a ... -
Molecular detection and study of Campylobacter and related microorganisms
(University of the Western Cape, 2010)Species of Campylobacter, Arcobacter and Helicobacter have been associated with various diseases in humans and animals; and chickens have been identified as a reservoir of these microorganisms. Two published techniques and ... -
The C-economy, nutritional benefits and symbiotic performance of dual inoculated Phaseolus vulgaris (L.) plants, under variable nutrient conditions
(University of the Western Cape, 2010)The tripartite symbiosis between Phaseolus vulgaris, arbuscular-mycorrhiza and the nodule bacteria, Rhizobia have been the focus of many studies ranging over a number of decades, however these studies have failed to answer ... -
Respiratory and photosynthetic C and N metabolism of nodulated Lupin roots during phosphorus deficiency
(University of the Western Cape, 2010)Growth of symbiotic legume hosts is P limited, because of the high energetic requirements associated with N2 fixation. Attempts to overcome P deficiency in soils where legumes are grown involve addition of P-based fertilisers. ... -
Genetic mapping of fruit quality traits in apple (malus x domestica borkh.)
(University of the Western Cape, 2011)Apple fruit quality is of utmost importance to apple farmers and breeders in the selection and commercialization of new cultivars. Fruit size, colour, texture, firmness and taste are all traits that affect the quality of ... -
Investigation of the interactions of retinoblastoma binding protein-6 with transcription factors p53 and Y-Box Binding Protein-1
(2011)Retinoblastoma Binding Protein 6 (RBBP6) is a 250 kDa multi-domain protein that has been implicated in diverse cellular processes including apoptosis, mRNA processing and cell cycle regulation. Many of these functions are ... -
The implementation of sub-typing techniques to determine the diversity of L. monocytogenes strains adapted to the food processing environment and their association with human listeriosis cases
(University of the Western Cape, 2011)Listeria monocytogenes has been established as a food-borne pathogen since the early 1980s and has become a big concern for the food industry and Public Health authorities (Doyle 2001; Oliveira et al. 2003; Capita et al. ... -
Isolation, identification and characterisation of novel actinobacteria from Zambian hot-springs
(2011)Actinomycetes are ubiquitous in many environments such as soil, activated sludge and water.Besides the genus Streptomyces, which has been extensively exploited, members of other genera including Micromonospora have been ... -
Structural and functional characterisation of a novel signalling molecule in Arabidopsis thaliana
(2011)Nitric Oxide (NO) influences a wide range of physiological processes in plants including growth and development, responses to abiotic and biotic stress and pathogen responses. NO binds to the heme group of the mammalian ... -
Manipulating biotic and abiotic factors to enhance the remediation of agri-industrial wastewater in pilot-scale constructed wetlands
(University of the Western Cape, 2012)As a consequence of various cellar activities the wine industry produces copious volumes of potentially hazardous wastewater each year. South Africa is one of the top ten wine-producing countries, making the successful ...