Dentine permeability and cytotoxicity of a dentine bonding agent

Abstract

The objectives of this study were to create an in vitro situation which closest resembled the clinical situation by inclusion of human dentine discs at a specified anatomic location as barrier and evaluating if comparable or graded perfusion could be identified as well as whether a link existed between dentine thickness and permeability. The other leg of the study was to evaluate the cytotoxicity of Xeno III, one of the newly released new generation dentine bonding agents and to evaluate its cytotoxicity in an in vitro pulp chamber device, determining which thickness of dentine would be able to protect the dental pulp from noxious substances in the bonding agent.