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A molecular investigation of the prevalence of suspected periodontopathogens and their association with preterm birth
(University of the Western Cape, 2010)
More than 20 million infants in the world (15.5 % of all births) are born with low birth weight. Ninety-five % of them are in developing countries. Oral colonization of Gramnegative anaerobes has been implicated as a risk ...
Molecular Characterisation of the Brassinosteroid, Phytosulfokine and cGMP-dependent Responses in Arabidopsis thaliana
(University of the Western Cape, 2010)
In this thesis, we have firstly cloned and expressed the domains that harbours the putative catalytic GC domain in these receptor molecules and demonstrate that these molecules can convert GTP to cGMP in vitro. Secondly, ...
Structural characterisation of the interaction between RBBP6 and the multifunctional protein YB-1
(University of the Western Cape, 2010)
As a means of further localising the interaction, truncated fragments derived from the C-terminal region of YB-1, were tested for their interaction with the RING finger domain of RBBP6 using three different assays: a ...
Identification of a transducin (beta)-like 3 protein as a potential biomarker of prediabetes from rat urine using proteomics
(University of the Western Cape, 2010)
Obesity is a globally increasing disease particularly in developing countries and among children. It is mainly caused by intake of diets high in fat and the lack of physical activity. Obesity is a risk factor for diseases ...
Molecular detection and study of Campylobacter and related microorganisms
(University of the Western Cape, 2010)
Species of Campylobacter, Arcobacter and Helicobacter have been associated with various diseases in humans and animals; and chickens have been identified as a reservoir of these microorganisms. Two published techniques and ...
Development and validation of Non-CODIS miniSTR genotyping systems suitable for forensic case work in South Africa
(University of the Western Cape, 2010)
The objective of this study was to develop and validate a six Non-CODIS miniSTR genotyping system and to determine its suitability for forensic casework in South Africa. In Non-CODIS miniSTR genotyping systems, smaller PCR ...
Development of Y-STR genotyping systems suitable for sexual assault cases in South Africa
(University of the Western Cape, 2010)
Sexual assault is a significant problem facing the South African society. In this context, efficient but also affordable genotyping systems are needed for positive identification of criminals in incidences of sexual violence. ...
Identification and characterisation of novel cellulolytic genes using metagenomics
(University of the Western Cape, 2010)
Metagenomics has been successfully used to discover novel enzymes from uncultured microorganisms in the environment. In this study, metagenomic DNA from a Malawian hot spring soil sample was used to construct a fosmid ...
Isolation and characterisation of esterases from thermophilic Actinomyces
(University of the Western Cape, 2010)
Alternative sources of fuel are required worldwide, and bio-ethanol is the leading candidate. Lignocellulosic biomass, a waste component of the agricultural industry, is a promising renewable source. Due to its complex ...
Identification and characterisation of hemicellulases from thermophilic Actinomycetes
(University of the Western Cape, 2010)
To ensure the sustainability of bioethanol production, major attention has been directed to develop feedstocks which provide an alternative to food-crop biomass. Lignocellulosic (LC) biomass, which is chiefly composed of ...