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Screening for subtate tolerant Geobacillus pallidus RAPc8 nitrile hydratase
(University of the Western Cape, 2009)
In this study G. pallidus RAPc8 NHase mutants were screened for reduced substrate inhibition compared to the wild type enzyme. Wild type and mutant enzymes were expressed and purified using hydrophobic interaction ...
Engineering homoaromatic substrate specificity into aliphatic-specific Geobacillus pallidus RAPc8 nitrile hydratase
(University of the Western Cape, 2007)
Geobacillus pallidus RAPc8 is a thermophilic nitrile-degrading isolate, obtained from thermal sediment samples of a New Zealand hot spring. The G. pallidus RAPc8 NHase gene has been cloned and expressed in E. coli. The ...
The diversity of key anabolic genes in antarctic hypolithons
(2009)
Antarctica is known for its pristine environments. A variety of unsuitable environmental conditions were once thought to render the continent unsuitable for sustaining life. However, metagenomic data have revealed a wealth ...
The structure of the nitrilase from Rhodococcus Rhodochrous J1: homology modeling and three-dimensional reconstruction
(University of the Western Cape, 2006)
The nitrilases are an important class of industrial enzymes that are found in all phyla. These enzymes are expressed widely in prokaryotes and eukaryotes. Nitrilases convert nitriles to corresponding acids and ammonia. ...
Screening extracts of indigenous South African plants for the presence of anti-cancer compounds
(University of the Western Cape, 2006)
Early man dabbled with the use of plant extracts to cure ailments. This practice has been passed down from generation to generation and today more than 50% of the world'sdrugs are natural products or derivatives thereof. ...
Phylogenetic diversity of nifH genes in Marion Island soil
(University of the Western Cape, 2006)
The microbial life of sub-Antarctic islands plays a key role in the islands ecosystem, with microbial activities providing the majority of nutrients available for primary production. Knowledge of microbial diversity is ...
Investigation of South African estuarine microbial species and genome diversity
(University of the Western Cape, 2006)
A study of the microbial diversity in sediments of the Great Berg River estuary is carried out using modern molecular phylogenetic methods. The aim of this study was to determine the effect of (pollution by) the effluents ...
Iterative helical real-space reconstruction of histone octamer tubular crystals and implications for the 30 nm chromatin fiber
(University of the Western Cape, 2006)
This thesis investigated the helical structure of core histone octamers to discover interacting surfaces and their relevance to the compaction of nucleosome arrays into the chromating fiber.
Microbial diversity of Antarctic Dry Valley mineral soil
(University of the Western Cape, 2004)
Antarctica provides some of the most extreme environments on earth. Low temperatures, low water availability and nutrient deficiency are contributing factors to the limited colonisation of Antarctic biotopes, particularly ...
The prevalence and survival of Campylobacter, Salmonella and Listeria species in poultry processing plant
(University of the Western Cape, 2004)
The organisms in this study were chosen due to their associations with foods and their potential as food borne pathogens. Food borne diseases are an import public health problem in most countries. Bacteria of the genera ...