Browsing Philosophiae Doctor - PhD (Biotechnology) by Author "Cowan, Don A."
Now showing items 1-6 of 6
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Characterisation of microbial communities associated with hypolithic environments in Antarctic Dry Valley soils
Khan, Nuraan (University of the Western Cape, 2008)The Eastern Antarctic Dry Valley region is a polar desert, where conditions of extreme aridity, high temperature fluctuations and high irradiation levels make it one of the most extreme environments on earth. Despite the ... -
Metagenomic approaches to gene discovery
Meyer, Quinton Christian (University of the Western Cape, 2006)The classical approach to gene discovery has been to culture micro-organisms demonstrating a specific enzyme activity and then to recover the gene of interest through shotgun cloning. The realization that these standard ... -
Microbial diversity and gene mining in Antarctic Dry Valley mineral soils
Smith, Jacques J. (University of the Western Cape, 2006)Soil communities are regarded as among the most complex and diverse assemblages of microorganisms with estimated bacterial numbers in the order of 109-1 cells.g. Studies on extreme soils however, have reported lower cell ... -
Mining a Chinese hyperthermophilic metagenome
Du Plessis, Morne Graham (University of the Western Cape, 2007)Metagenomic sequencing of environmental samples provide direct access to genomic information of organisms within the respective environments. This sequence information represents a significant resource for the identification ... -
The relationship between structure and thermostability of a nitrile hydratase from Goebacillus pallidus RAPc8
Van Wyk, Jennifer Caroline (University of the Western Cape, 2008)Nitrile hydratases (NHases) are very important biocatalysts for the enzymatic conversion of nitriles to industrially important amides such as acrylamide and nicotinamide. An “ideal” NHase should fulfil several essential ... -
Structure, enzymology and genetic engineering of Bacillus sp. RAPc8 nitrile hydratase
Tsekoa, Tsepo L. (University of the Western Cape, 2005)Microbial nitrile hydratases (NHases) are important industrial enzymes that catalyse the conversion of nitriles to the corresponding amides. A thermostable, cobalt-type Bacillus sp. RAPc8 NHase was previously cloned and ...