Browsing Magister Scientiae - MSc (Biotechnology) by Title
Now showing items 225-244 of 270
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Recombinant expression and full backbone assignment of the human DWNN using heteronuclear NMR
(University of the Western Cape, 2005)The cellular levels of a number of proteins have been found to be regulated by the ubiquitin-proteasome pathway. In this pathway, proteins are covalently tagged (“ubiquitinated”) by ubiquitin, which acts as a signal for ... -
Recombinant expression of cytochrome P450-2D6 and its application in tamoxifen metabolism
(University of the Western Cape, 2018)Breast cancer is regarded as the most common form of cancer in women and it comprises of approximately 23 % of female cancers, while affecting women at any age range. For oestrogen receptor positive patients, tamoxifen is ... -
Recombinant expression of the pRb- and p53-interacting domains from the human RBBP6 protein for in vitro binding studies
(University of the Western Cape, 2004)This thesis describes the cloning and recombinant expression of domains from the human RBBP6 protein for future in vitro binding studies with pRb and p53. RBBP6 is a splicing-associated protein that is known to interact ... -
Recombinant expression of the pRb- and p53-interacting domains from the human RBBP6 protein for in vitro binding studies
(University of the Western Cape, 2004)This thesis describes the cloning and recombinant expression of domains from the human RBBP6 protein for future in vitro binding studies with pRb and p53. RBBP6 is a splicing-associated protein that is known to interact ... -
Reconstruction of major male and female lineages of the kensington Muslim community
(University of the Western Cape, 2010)The earliest Cape Muslims were brought to the Cape from Africa and Asia from 1652 to 1834. They were part of an involuntary migration of slaves, political prisoners and convicts, and they contributed in the ethnic diversity ... -
Reconstruction of major male and female lineages of the Strand Muslim community
(University of the Western Cape, 2010)Initially, a pilot study was carried out in order to reconstruct the major paternal and maternal lineages of the Muslim population living in the Cape metropolitan area. The Study has shown the ability of molecular genetic ... -
Response of microbial communities to synthetic winery wastewater in biological sand filters
(University of the Western Cape, 2017)There has been a significant increase in the use of constructed wetlands globally for the bioremediation of wastewater (Welz et al., 2011). In South Africa, the wine industry generates more than one billion litres of ... -
Responses of maize Antioxidant Enzymes to drought stress
(University of the Western Cape, 2019)Maize (Zea mays L) was subjected to drought stress for 28 days. The effects of the drought stress on growth, H2O2 content and lipid peroxidation were investigated and the activities of antioxidant enzymes [superoxide ... -
Responses of maize antioxidant enzymes to drought stress
(University of the Western Cape, 2019)Maize (Zea mays L) was subjected to drought stress for 28 days. The effects of the drought stress on growth, H2O2 content and lipid peroxidation were investigated and the activities of antioxidant enzymes [superoxide ... -
Sample preparation methods and molecular based detection for the rapid isolation and identification of Listeria monocytogenes in food samples
(University of the Western Cape, 2006)Listeria monocytogenes is a Gram-positive bacterium responsible for listeriosis, a food-borne disease, which may result in severe illness and possible death. The importance of L. monocytogenes as a food-borne pathogen has ... -
Screening bacterial symbionts of marine invertebrates for ribosomally synthesized natural products
(University of the Western Cape, 2016)Pharmaceutical research and development strategies rely on the constant discovery of novel natural products as potential drugs. Recent studies have shown that the microorganisms associated with sponges are the true producers ... -
Screening extracts of indigenous South African plants for the presence of anti-cancer compounds
(University of the Western Cape, 2006)Early man dabbled with the use of plant extracts to cure ailments. This practice has been passed down from generation to generation and today more than 50% of the world'sdrugs are natural products or derivatives thereof. ... -
Screening for fluorescent and chromoproteins from South African sea anemones
(University of the Western Cape, 2012)Sea anemones (Order Actinaria) are a diverse order from the Class Anthozoa. They are found in all marine habitats at all depths and their symbiotic relationships play an important role in energy transfers especially in the ... -
Screening for subtate tolerant Geobacillus pallidus RAPc8 nitrile hydratase
(University of the Western Cape, 2009)In this study G. pallidus RAPc8 NHase mutants were screened for reduced substrate inhibition compared to the wild type enzyme. Wild type and mutant enzymes were expressed and purified using hydrophobic interaction ... -
Screening of marine bacteria as a source of bioactive secondary metabolites
(University of Western Cape, 2021)Marine invertebrate associated (MIA) bacteria are an important source of bioactive secondary metabolites with the potential to address the current anti-microbial resistance crisis experienced globally. Secondary metabolites ... -
Screening of natural products and alkylating agents for antineoplastic activity
(2007)Apoptosis is a process in which a cell programmes its own death. It is a highly organized physiological mechanism in which injured or damaged cells are destroyed. Apart from physiological stimuli however, exogenous factors ... -
Screening of natural products and alkylating agents for antineoplastic activity
(University of the Western Cape, 2007)Background and objectives: Apoptosis is a process in which a cell programmes its own death. It is a highly organized physiological mechanism in which injured or damaged cells are destroyed. Apart from physiological stimuli ... -
Screening of plant-mediated nanoparticles for antifungal activity
(University of the Western Cape, 2020)Nanotechnology is spreading rapidly across the world as an extremely powerful technology. Nanoscience and nanotechnology are innovative scientific advancements that have been introduced only in this century. Nanotechnology ... -
Seasonal dynamics of edaphic bacterial communities in the hyper-arid Namib desert
(University of the Western Cape, 2014)The aim of this study is to investigate the edaphic bacterial community dynamics over short (57 days) and long-term (1 year) sampling intervals using an extensive sampling strategy in combination with community fingerprinting ... -
Signalling molecule “calcium” improves germination and growth of Sorghum bicolor seedlings under salt stress
(University of the Western Cape, 2021)Abiotic stress, mainly in the form of extreme temperatures, drought and salinity has caused major crop losses worldwide, putting a severe strain on agriculture. Salinity severely limits plant growth and productivity and ...