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dc.contributor.advisorBaker, Priscilla G.L.
dc.contributor.authorMangombo, Zelo
dc.date.accessioned2023-06-12T13:01:52Z
dc.date.available2023-06-12T13:01:52Z
dc.date.issued2010
dc.identifier.urihttp://hdl.handle.net/11394/10151
dc.description>Magister Scientiae - MScen_US
dc.description.abstractThe aim of this project was to develop a biosensor on a modified boron-doped diamond (BDD) platform in order to determine L-phenylalanine (L-Phe) levels in the aqueous media and real samples. A BDD electrode modified with polyaniline (PANI) film doped with polyvinyl sulphonate (PVS), containing in-situ deposited tyrosinase (Tyr) enzyme was studied with special emphasis on the detection of L-phenylalanine (L-Phe). The PANI-PVS film was electrochemically coated on the BDD surface by cyclic voltammetry (CV) at 50 mV s-1 (versus Ag/AgCI). An increase in current density with increasing number of cycles was an indication of the polymer growth. The morphologies of the composite films were characterized by scanning electron microscopy (SEM).en_US
dc.language.isoenen_US
dc.publisherUniversity of the Western Capeen_US
dc.subjectPolyanilineen_US
dc.subjectElectrochemical enzyme biosensoren_US
dc.subjectChemistryen_US
dc.subjectCyclic voltammetryen_US
dc.subjectLipropoteinen_US
dc.titleThe development of l-phenylalanine biosensor using boron-doped diamond (bdd) electrode modified with poly aniline-polyvinyl sulphonate composite filmen_US
dc.rights.holderUniversity of the Western Capeen_US


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