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dc.contributor.advisorReeves, Gail
dc.contributor.authorKhunou, Angeline
dc.date.accessioned2023-06-13T12:02:52Z
dc.date.available2023-06-13T12:02:52Z
dc.date.issued2004
dc.identifier.urihttp://hdl.handle.net/11394/10215
dc.descriptionMagister Scientiae (Biodiversity and Conservation Biology)en_US
dc.description.abstractDuring the course of this thesis, AFLP technique was successfully applied to the Aizoaceae taxa. This study involved a substantial component of laboratory work including generating and analysing AFLP data, the operation of an ABI377 automated DNA sequencer, and use of GeneScan and Genotyper software (Applied Biosystems). The initial motivation for this study was to reconstruct relationships between species of the genus Carpobrotus. However, it soon became clear that this would be impossible via the use of DNA sequence data. This was due to the inability to find DNA regions with sufficient variable characters for phylogenetic reconstruction. Therefore applying the AFLP fingerprinting technique to the question of species-level relationships in Carpobrotus resulted in individuals of the same species not forming distinct groups. This has subsequently led to the conclusion that gene flow is common across species boundaries in Carpobrotus as a result of hybridisation. An alternative ved may be that current species delimitations in this group are not well defined. These results also concord well with isozyme studies taxa in the Mediterranean Basin. Increased sampling of species other than C. acinaciformis and C. edulis would allow further investigation into the genetic delimitation and affinities of Carpobrotus, and shed light on the In the biotechnological age the use of molecular techniques has has the potential to greatly improve the bio-prospecting process. This has particular relevance to plants with medicinal properties, such as Carpobrotus, whereby targeted selection of genotypes with well-characterised medicinal properties may increase efficiency. Genetic and phylogenetic information can also help to identify closely related species and genera that may share similar metabolic pathways and properties. These issues are quickly coming to the forefront in the new South Africa, with the opening up of traditional knowledge systems, and subsequent integration and contact with the biotechnology community. 79en_US
dc.language.isoenen_US
dc.publisherUniversity of the Western Capeen_US
dc.subjectAizoaceaeen_US
dc.subjectAFLPsen_US
dc.subjectAnti-microbial activityen_US
dc.subjectCarpobrotusen_US
dc.subjectPhylogenyen_US
dc.subjectPopulationsen_US
dc.subjectGenetic diversityen_US
dc.subjectHybridizationen_US
dc.subjectRuschioideaeen_US
dc.subjectSouth Africaen_US
dc.titleSpecies delimitation and assessment of genetic variability in Carpobrotus (Aizoaceae): evidence from amplified fragment length polymorphismsen_US
dc.rights.holderUniversity of the Western Capeen_US


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