Browsing Philosophiae Doctor - PhD (Biotechnology) by Title
Now showing items 87-98 of 98
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Solution structure of the RING finger domain from the human splicing-associated protein RBBP6 using heteronuclear Nuclear Magnetic Resonance (NMR) spectroscopy
(2009)Retinoblastoma-binding protein 6 (RBBP6) is a multi-domain human protein known to play a role in mRNA splicing, cell cycle control and apoptosis. The protein interacts with tumour suppressor proteins p53 and pRb and recent ... -
Structural and functional characterisation of a novel signalling molecule in Arabidopsis thaliana
(2011)Nitric Oxide (NO) influences a wide range of physiological processes in plants including growth and development, responses to abiotic and biotic stress and pathogen responses. NO binds to the heme group of the mammalian ... -
Structural and functional studies of XvPrx2, a type II peroxiredoxin protein from the resurrection plant xerophyta viscosa
(University of the Western Cape, 2012)XvPrx2 is a 1-Cys-containing member of the Prx5 subfamily of peroxiredoxins isolated from the resurrection plant Xerophyta viscosa. It is reported to be up-regulated during periods of desiccation and to protect nucleic ... -
Structural characterisation of the interaction between RBBP6 and the multifunctional protein YB-1
(University of the Western Cape, 2010)As a means of further localising the interaction, truncated fragments derived from the C-terminal region of YB-1, were tested for their interaction with the RING finger domain of RBBP6 using three different assays: a ... -
Structure, enzymology and genetic engineering of Bacillus sp. RAPc8 nitrile hydratase
(University of the Western Cape, 2005)Microbial nitrile hydratases (NHases) are important industrial enzymes that catalyse the conversion of nitriles to the corresponding amides. A thermostable, cobalt-type Bacillus sp. RAPc8 NHase was previously cloned and ... -
Studies on the population genetics of Euphausiids: a comparison of patterns in plagic taxa displaying different distributions and life-histories
(University of the Western Cape, 2006)The systematic and population genetic relationships were characterised for three ecologically related euphausiid species: Euphausia lucens, E. recurva and E. vallentini. These species have different geographical distributions ... -
The effects of high temperature stress on the enzymatic antioxidant system in Zea mays
(University of the Western Cape, 2017)High temperature stress is synonymous with the attenuation of plant growth, metabolism and eventually death resulting in major loss of crop productivity worldwide. Part of the metabolic perturbations associated with heat ... -
Transcription regulation and candidate diagnostic markers of esophageal cancer
(University of the Western Cape, 2009)Esophageal cancer (EC) ranks among the ten most frequent cancers worldwide. Mortality rates associated with EC are very similar to the incidence rates due to the relatively late stage of diagnosis and the poor efficacy of ... -
A transcriptome analysis of apple (Malus x domestica Borkh.) cv ‘golden delicious’ fruit during fruit growth and development
(2013)The growth and development of apple (Malus x domestica Borkh.) fruit occurs over a period of about 150 days after anthesis to full ripeness. During this period morphological and physiological changes occur defining fruit ... -
Tumour specific targeted in vitro theranostics application of fabricated nanostructures in a multi-drug resistant ovarian carcinoma cell line
(University of the Western Cape, 2013)Ovarian cancer is called the “Silent Killer” as it is often diagnosed in advanced stages of the disease or misdiagnosed which ends with a poor prognostic outcome for the patient. A high rate of disease relapse, a high ... -
Validation and application of a highly discriminating and rapid 10-locus Y-STR DNA profiling system
(University of the Western Cape, 2019)DNA profiling the male specific region on the Y-chromosome is fundamental to forensic practise. Its recognised as a powerful analytical tool for investigation of sexual assault when the DNA evidence is highly admixed. ... -
A yeast 2-hybrid screen to identify and characterize interaction partners of the cancer associated protein retinoblastoma binding protein 6
(2009)Retinoblastoma binding protein 6 (RBBP6) is a 250 kDa protein that is implicated in mRNA processing and ubiquitination functions and has been shown to be highly up-regulated in a number of cancers. In humans and mice,RBBP6 ...