Browsing Magister Scientiae - MSc (Biotechnology) by Title
Now showing items 234-253 of 270
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Sample preparation methods and molecular based detection for the rapid isolation and identification of Listeria monocytogenes in food samples
(University of the Western Cape, 2006)Listeria monocytogenes is a Gram-positive bacterium responsible for listeriosis, a food-borne disease, which may result in severe illness and possible death. The importance of L. monocytogenes as a food-borne pathogen has ... -
Screening bacterial symbionts of marine invertebrates for ribosomally synthesized natural products
(University of the Western Cape, 2016)Pharmaceutical research and development strategies rely on the constant discovery of novel natural products as potential drugs. Recent studies have shown that the microorganisms associated with sponges are the true producers ... -
Screening extracts of indigenous South African plants for the presence of anti-cancer compounds
(University of the Western Cape, 2006)Early man dabbled with the use of plant extracts to cure ailments. This practice has been passed down from generation to generation and today more than 50% of the world'sdrugs are natural products or derivatives thereof. ... -
Screening for fluorescent and chromoproteins from South African sea anemones
(University of the Western Cape, 2012)Sea anemones (Order Actinaria) are a diverse order from the Class Anthozoa. They are found in all marine habitats at all depths and their symbiotic relationships play an important role in energy transfers especially in the ... -
Screening for subtate tolerant Geobacillus pallidus RAPc8 nitrile hydratase
(University of the Western Cape, 2009)In this study G. pallidus RAPc8 NHase mutants were screened for reduced substrate inhibition compared to the wild type enzyme. Wild type and mutant enzymes were expressed and purified using hydrophobic interaction ... -
Screening of marine bacteria as a source of bioactive secondary metabolites
(University of Western Cape, 2021)Marine invertebrate associated (MIA) bacteria are an important source of bioactive secondary metabolites with the potential to address the current anti-microbial resistance crisis experienced globally. Secondary metabolites ... -
Screening of natural products and alkylating agents for antineoplastic activity
(2007)Apoptosis is a process in which a cell programmes its own death. It is a highly organized physiological mechanism in which injured or damaged cells are destroyed. Apart from physiological stimuli however, exogenous factors ... -
Screening of natural products and alkylating agents for antineoplastic activity
(University of the Western Cape, 2007)Background and objectives: Apoptosis is a process in which a cell programmes its own death. It is a highly organized physiological mechanism in which injured or damaged cells are destroyed. Apart from physiological stimuli ... -
Screening of plant-mediated nanoparticles for antifungal activity
(University of the Western Cape, 2020)Nanotechnology is spreading rapidly across the world as an extremely powerful technology. Nanoscience and nanotechnology are innovative scientific advancements that have been introduced only in this century. Nanotechnology ... -
Seasonal dynamics of edaphic bacterial communities in the hyper-arid Namib desert
(University of the Western Cape, 2014)The aim of this study is to investigate the edaphic bacterial community dynamics over short (57 days) and long-term (1 year) sampling intervals using an extensive sampling strategy in combination with community fingerprinting ... -
Signalling molecule “calcium” improves germination and growth of Sorghum bicolor seedlings under salt stress
(University of the Western Cape, 2021)Abiotic stress, mainly in the form of extreme temperatures, drought and salinity has caused major crop losses worldwide, putting a severe strain on agriculture. Salinity severely limits plant growth and productivity and ... -
Silica Coated Core-Shell Quantum Dot-based Electro-Immunosensor for Interferon Gamma TB Disease Biomarker
(University of the Western Cape, 2020)Tuberculosis (TB) is a disease that results from infection by Mycobacterium tuberculosis, which is regarded the most common infecting organism. TB has killed countless numbers of people particularly in underdeveloped ... -
Single nucleotide polymorphism association studies of ABCA13 and ABHD11 genes and the bioinformatics analysis of the autism candidate genes localized on chromosome 7
(University of the Western Cape, 2016)Autism, Aspergers Syndrome and Pervasive Developmental Delay-Not Otherwise Specified (PDD-NOS), among others, fall under an umbrella of disorders known as Autism Spectrum Disorder. Twin studies show that autism is a highly ... -
South African medicinal plant extracts used in the treatment of fungal infections.
(University of the Western Cape, 2023)The incidence of fungal skin infections is increasing at an alarming rate, especially in people with underlying immunosuppressive conditions. Currently, the most used methods of treatment are in the azole group of synthetic ... -
Spartial distribution of cyanobacterial phylotypes in Antarctic Dry Valley soil biotopes
(University of the Western Cape, 2007)Recent advances in moleculr methods have enabled the analysis of cyanobacterial diversity using PCR- based approaches. Snap-shot techniques such as 16S rDNA library construction and DGGE have allowed for increased access ... -
SSR-based genetic mapping of QTLs determining chilling requirements for time of initial vegetative budbreak in domesticated apple (Malus x domestica Borkh.) cultivar ‘Anna’ x ‘Austin’
(University of the Western Cape, 2012)The Rosaceae family contains major temperate crops such as the domesticated apple(Malus x domestica Borkh.), peach (Prunus persica L. Batsch) and European pear (Pyrus communis L.). However, despite its evident economic ... -
Structural analysis of induced mutagenesis A’ protein from mycobacterium tuberculosis and of a thermophillic GH9 cellulase
(University of the Western Cape, 2014)The three-dimensional structures of proteins are important in understanding their function and interaction with ligands and other proteins. In this work, the structures of two proteins, ImuA’ from mycobacterium tuberculosis ... -
Structural Analysis of Induced Mutagenesis Protein B from Mycobacterium tuberculosis Jeremy
(University of the Western Cape, 2016)Knowing the three-dimensional structure of a protein may be useful in understanding its function. In this study, induced mutagenesis protein B (ImuB) from Mycobacterium tuberculosis was analyzed using molecular biology and ... -
Structural characterisation of the interaction between RBBP6 and the multifunctional protein YB-l
(University of the Western Cape, 2010)Retinoblastoma binding protein 6 (RBBP6) is a 250 kDa RING finger-containing protein whose function is known to be mediated through interaction with other proteins. RBBP6 plays a role in the regulation of the tumour ... -
The structure of the nitrilase from Rhodococcus Rhodochrous J1: homology modeling and three-dimensional reconstruction
(University of the Western Cape, 2006)The nitrilases are an important class of industrial enzymes that are found in all phyla. These enzymes are expressed widely in prokaryotes and eukaryotes. Nitrilases convert nitriles to corresponding acids and ammonia. ...