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dc.contributor.advisorRees, D.J.G.
dc.contributor.advisorChristoffels, Alan
dc.contributor.authorChikwambi, Zedias
dc.date.accessioned2015-02-09T11:51:23Z
dc.date.available2015-02-09T11:51:23Z
dc.date.issued2013
dc.identifier.urihttp://hdl.handle.net/11394/3952
dc.descriptionPhilosophiae Doctor - PhDen_US
dc.description.abstractThe growth and development of apple (Malus x domestica Borkh.) fruit occurs over a period of about 150 days after anthesis to full ripeness. During this period morphological and physiological changes occur defining fruit quality. These changes are a result of spatial and temporal patterns of gene expression during fruit development as regulated by environmental, genetic and environmental-by-genetic factors. A number of previous studies partially characterised the transcriptomes of apple leaf, fruit pulp, whole fruit, and peel plus pulp tissues, using cDNA micro arrays and other PCR based technologies. These studies, however, remain limited in throughput and specificity for transcripts of low abundance. Hence, the aim of this project was to apply a high throughput technique to characterise the full mRNA transcriptome of the ‘Golden Delicious’ fruit peels and pulp tissues in order to understand the molecular mechanisms underlying the morphophysiological changes that occur during fruit development.en_US
dc.language.isoenen_US
dc.subjectApple fruiten_US
dc.subjectFruit pulpen_US
dc.subjectFruit peelen_US
dc.subjectTranscriptomeen_US
dc.subjectDifferential gene expressionen_US
dc.subjectFull-length transcript reconstructionen_US
dc.subjectFruit developmenten_US
dc.subjectGenomicsen_US
dc.subjectRNA-seqen_US
dc.subjectBioinformaticsen_US
dc.titleA transcriptome analysis of apple (Malus x domestica Borkh.) cv ‘golden delicious’ fruit during fruit growth and developmenten_US
dc.rights.holderUniversity of the Western Capeen_US


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