Antimicrobial efficacy of Nonchlorhexidine non-Alcohol containing mouthrinses

Abstract

Aim: to compare antimicrobial efficacy of three different non-chlorhexidine non-alcohol containing mouthrinses. Objectives: to test antimicrobial efficacy of Colgate total®, Biobalance mouthwash® and Listerine Zero® against Streptococcus mutans, Staphylococcus aureus, Candida albicans, Enterococcus faecalis and the aerobic and facultative anaerobic organisms cultured from the collected oral saliva. Materials and methods: fourteen saliva samples were collected from staff members who fit the inclusion criteria along with fourteen pure cultures of each of the tested microorganisms. All samples were cultured in agar plates. Four sterile, 5mm discs were used for each plate, each representing one of the tested mouthrinses. Each disc was immersed for one minute and then deposited on sterile gauze to remove excess fluid. Then plates were incubated for 24 hours at 37 ? Celsius. Inhibition zones created around the discs were measured using an electronic caliber. Results: most of the tested mouthrinses showed antimicrobial efficacy against tested microorganism. Differences between them were statistically significant (p. value =0.0001). The order in terms of antimicrobial efficacy was Colgate total®; Biobalance mouthwash®; Listerine Zero®.