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dc.contributor.advisorLudidi, Ndiko Ndomelele
dc.contributor.authorLilelo, Andile
dc.date.accessioned2020-02-27T12:25:54Z
dc.date.available2020-02-27T12:25:54Z
dc.date.issued2019
dc.identifier.urihttp://hdl.handle.net/11394/7174
dc.description>Magister Scientiae - MScen_US
dc.description.abstractMaize (Zea mays L) was subjected to drought stress for 28 days. The effects of the drought stress on growth, H2O2 content and lipid peroxidation were investigated and the activities of antioxidant enzymes [superoxide dismutase (SOD), ascorbate peroxidase (APX) and catalase (CAT)], were measured. Reverse transcription quantitative PCR (RT-qPCR) was used to measure relative expression of APX and CAT genes in response to drought stress. Maize biomass was significantly reduced and cell death was higher in drought-stressed plants. Similarly, the activities of antioxidant enzymes (SOD, APX and CAT) increased in drought-stressed plants. Lipid peroxidation (measured as malondialdehyde content) and hydrogen peroxide content increased in drought-stressed plants. The expression of APX genes in drought-stressed (water-deprived) leaves was significantly higher than the well-watered control. CAT gene expression showed differential response between the leaves and roots.en_US
dc.language.isoenen_US
dc.publisherUniversity of the Western Capeen_US
dc.subjectMaizeen_US
dc.subjectDroughten_US
dc.subjectReactive oxygen species (ROS)en_US
dc.subjectOxidative stressen_US
dc.subjectAntioxidant enzymesen_US
dc.subjectSuperoxide dismutase (SOD)en_US
dc.subjectCatalase (CAT)en_US
dc.subjectAscorbate peroxidase (APX)en_US
dc.subjectRelative gene expressionen_US
dc.subjectCell deathen_US
dc.titleResponses of maize Antioxidant Enzymes to drought stressen_US
dc.rights.holderUniversity of the Western Capeen_US


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