Browsing Faculty of Natural Science by Author "Dept. of Biotechnology"
Now showing items 41-55 of 55
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Physiological and cellular characterization of a plant natriuretic peptide
Maqungo, Monique Nonceba (University of the Western Cape, 2005)Plants in the field are exposed to multiple stresses and their response to these various stresses determines their capacity to survive. Plants can use multiple signaling pathways and signals to mediate their response; for ... -
The prevalence and survival of Campylobacter, Salmonella and Listeria species in poultry processing plant
Mabogo, Rudzani David Lesly (University of the Western Cape, 2004)The organisms in this study were chosen due to their associations with foods and their potential as food borne pathogens. Food borne diseases are an import public health problem in most countries. Bacteria of the genera ... -
Quantified assessment to enhance student learning in the sciences at UWC
Lombard, Ronell (University of the Western Cape, 2005)This project discusses whether the British prototype questionnaire called the Assessment Experience Questionnaire (AEQ) could be standardized as a quantifier of assessment and be used at a multicultural institute such as ... -
Recombinant expression and full backbone assignment of the human DWNN using heteronuclear NMR
Faro, Andrew (University of the Western Cape, 2005)The cellular levels of a number of proteins have been found to be regulated by the ubiquitin-proteasome pathway. In this pathway, proteins are covalently tagged (“ubiquitinated”) by ubiquitin, which acts as a signal for ... -
Recombinant expression of the pRb- and p53-interacting domains from the human RBBP6 protein for in vitro binding studies
Ndabambi, Nonkululeko (University of the Western Cape, 2004)This thesis describes the cloning and recombinant expression of domains from the human RBBP6 protein for future in vitro binding studies with pRb and p53. RBBP6 is a splicing-associated protein that is known to interact ... -
The relationship between structure and thermostability of a nitrile hydratase from Goebacillus pallidus RAPc8
Van Wyk, Jennifer Caroline (University of the Western Cape, 2008)Nitrile hydratases (NHases) are very important biocatalysts for the enzymatic conversion of nitriles to industrially important amides such as acrylamide and nicotinamide. An “ideal” NHase should fulfil several essential ... -
Sample preparation methods and molecular based detection for the rapid isolation and identification of Listeria monocytogenes in food samples
Rip, Diane (University of the Western Cape, 2006)Listeria monocytogenes is a Gram-positive bacterium responsible for listeriosis, a food-borne disease, which may result in severe illness and possible death. The importance of L. monocytogenes as a food-borne pathogen has ... -
Screening extracts of indigenous South African plants for the presence of anti-cancer compounds
Essack, Magbubah (University of the Western Cape, 2006)Early man dabbled with the use of plant extracts to cure ailments. This practice has been passed down from generation to generation and today more than 50% of the world'sdrugs are natural products or derivatives thereof. ... -
Screening for subtate tolerant Geobacillus pallidus RAPc8 nitrile hydratase
Mketsu, Moses Clive Masisange (University of the Western Cape, 2009)In this study G. pallidus RAPc8 NHase mutants were screened for reduced substrate inhibition compared to the wild type enzyme. Wild type and mutant enzymes were expressed and purified using hydrophobic interaction ... -
Screening of natural products and alkylating agents for antineoplastic activity
Kanyanda, Stonard Sofiel Elisa (University of the Western Cape, 2007)Background and objectives: Apoptosis is a process in which a cell programmes its own death. It is a highly organized physiological mechanism in which injured or damaged cells are destroyed. Apart from physiological stimuli ... -
Spartial distribution of cyanobacterial phylotypes in Antarctic Dry Valley soil biotopes
Keyster, Marshall (University of the Western Cape, 2007)Recent advances in moleculr methods have enabled the analysis of cyanobacterial diversity using PCR- based approaches. Snap-shot techniques such as 16S rDNA library construction and DGGE have allowed for increased access ... -
Structural characterisation of the interaction between RBBP6 and the multifunctional protein YB-1
Muleya, Victor (University of the Western Cape, 2010)As a means of further localising the interaction, truncated fragments derived from the C-terminal region of YB-1, were tested for their interaction with the RING finger domain of RBBP6 using three different assays: a ... -
Structure, enzymology and genetic engineering of Bacillus sp. RAPc8 nitrile hydratase
Tsekoa, Tsepo L. (University of the Western Cape, 2005)Microbial nitrile hydratases (NHases) are important industrial enzymes that catalyse the conversion of nitriles to the corresponding amides. A thermostable, cobalt-type Bacillus sp. RAPc8 NHase was previously cloned and ... -
The structure, function and engineering of a thermostable nitrile hydratase
Mavengere, William Nyasha (University of the Western Cape, 2008)Nitrile hydratases (NHases) are enzymes that catalyse the conversion of organocyanides to amides via a non-hydrolytic hydration reaction. They are industrially relevant enzymes, currently used in the manufacture of ... -
Systematic approach to protein crystallization: emphasis on Vaccinia virus complement control protein (VCP)
Adusei-Danso, Felix (University of the Western Cape, 2006)This work examined the systematic approach to protein crystallization, exploring some of the techniques that have been developed to enhance the success rate of crystallization. The work was centered on two proteins; namely ...