In vitro antibacterial activity of three root canal sealers against Enterococcus Faecalis
Mukorera, Tafadzwa Fraderick
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The goal of root canal treatment is to eradicate microorganisms in the root canal system of the tooth. However; it has been found that no method of tooth preparation is efficient in eliminating all microorganisms present in root canals. Therefore, obturation materials with anti-microbial properties are advantageous, so that any residual microorganisms in the root system of the tooth can be eliminated. Therefore, the aim of the study was to assess the antimicrobial effect of 3 endodontic sealers: Sealapex™, EndoREZ™ and Guttaflow bioseal™ against Enterococcus faecalis. The Direct Contact test was used to assess the antibacterial effect of the 3 sealers against E. Faecalis. Sample size was n=50 per sealer. The survival of bacteria was assessed by culturing aliquots of 100 μL onto Tryptic Soy Agar plates after 10-fold serial dilutions. After incubation for 24 hours at 37⁰C, colonies on the plates were counted, and the CFU/mL was calculated. The experiments were performed in triplicates. Testing after setting enabled the assessment of the antimicrobial activity of aged sealers after 7 days, 14 days, 21 days and 28 days. All 3 sealers displayed evidence of antibacterial activity against E. Faecalis with various degrees of antibacterial activity at day 0, 7, 14, 21 and 28. Antibacterial activity was displayed by all 3 sealers against E. Faecalis which will have an effect on entombed bacteria.