Browsing Magister Scientiae - MSc (Biotechnology) by Title
Now showing items 230-249 of 262
-
Screening for subtate tolerant Geobacillus pallidus RAPc8 nitrile hydratase
(University of the Western Cape, 2009)In this study G. pallidus RAPc8 NHase mutants were screened for reduced substrate inhibition compared to the wild type enzyme. Wild type and mutant enzymes were expressed and purified using hydrophobic interaction ... -
Screening of marine bacteria as a source of bioactive secondary metabolites
(University of Western Cape, 2021)Marine invertebrate associated (MIA) bacteria are an important source of bioactive secondary metabolites with the potential to address the current anti-microbial resistance crisis experienced globally. Secondary metabolites ... -
Screening of natural products and alkylating agents for antineoplastic activity
(2007)Apoptosis is a process in which a cell programmes its own death. It is a highly organized physiological mechanism in which injured or damaged cells are destroyed. Apart from physiological stimuli however, exogenous factors ... -
Screening of natural products and alkylating agents for antineoplastic activity
(University of the Western Cape, 2007)Background and objectives: Apoptosis is a process in which a cell programmes its own death. It is a highly organized physiological mechanism in which injured or damaged cells are destroyed. Apart from physiological stimuli ... -
Screening of plant-mediated nanoparticles for antifungal activity
(University of the Western Cape, 2020)Nanotechnology is spreading rapidly across the world as an extremely powerful technology. Nanoscience and nanotechnology are innovative scientific advancements that have been introduced only in this century. Nanotechnology ... -
Seasonal dynamics of edaphic bacterial communities in the hyper-arid Namib desert
(University of the Western Cape, 2014)The aim of this study is to investigate the edaphic bacterial community dynamics over short (57 days) and long-term (1 year) sampling intervals using an extensive sampling strategy in combination with community fingerprinting ... -
Signalling molecule “calcium” improves germination and growth of Sorghum bicolor seedlings under salt stress
(University of the Western Cape, 2021)Abiotic stress, mainly in the form of extreme temperatures, drought and salinity has caused major crop losses worldwide, putting a severe strain on agriculture. Salinity severely limits plant growth and productivity and ... -
Silica Coated Core-Shell Quantum Dot-based Electro-Immunosensor for Interferon Gamma TB Disease Biomarker
(University of the Western Cape, 2020)Tuberculosis (TB) is a disease that results from infection by Mycobacterium tuberculosis, which is regarded the most common infecting organism. TB has killed countless numbers of people particularly in underdeveloped ... -
Single nucleotide polymorphism association studies of ABCA13 and ABHD11 genes and the bioinformatics analysis of the autism candidate genes localized on chromosome 7
(University of the Western Cape, 2016)Autism, Aspergers Syndrome and Pervasive Developmental Delay-Not Otherwise Specified (PDD-NOS), among others, fall under an umbrella of disorders known as Autism Spectrum Disorder. Twin studies show that autism is a highly ... -
South African medicinal plant extracts used in the treatment of fungal infections.
(University of the Western Cape, 2023)The incidence of fungal skin infections is increasing at an alarming rate, especially in people with underlying immunosuppressive conditions. Currently, the most used methods of treatment are in the azole group of synthetic ... -
Spartial distribution of cyanobacterial phylotypes in Antarctic Dry Valley soil biotopes
(University of the Western Cape, 2007)Recent advances in moleculr methods have enabled the analysis of cyanobacterial diversity using PCR- based approaches. Snap-shot techniques such as 16S rDNA library construction and DGGE have allowed for increased access ... -
SSR-based genetic mapping of QTLs determining chilling requirements for time of initial vegetative budbreak in domesticated apple (Malus x domestica Borkh.) cultivar ‘Anna’ x ‘Austin’
(University of the Western Cape, 2012)The Rosaceae family contains major temperate crops such as the domesticated apple(Malus x domestica Borkh.), peach (Prunus persica L. Batsch) and European pear (Pyrus communis L.). However, despite its evident economic ... -
Structural analysis of induced mutagenesis A’ protein from mycobacterium tuberculosis and of a thermophillic GH9 cellulase
(University of the Western Cape, 2014)The three-dimensional structures of proteins are important in understanding their function and interaction with ligands and other proteins. In this work, the structures of two proteins, ImuA’ from mycobacterium tuberculosis ... -
Structural Analysis of Induced Mutagenesis Protein B from Mycobacterium tuberculosis Jeremy
(University of the Western Cape, 2016)Knowing the three-dimensional structure of a protein may be useful in understanding its function. In this study, induced mutagenesis protein B (ImuB) from Mycobacterium tuberculosis was analyzed using molecular biology and ... -
Structural characterisation of the interaction between RBBP6 and the multifunctional protein YB-l
(University of the Western Cape, 2010)Retinoblastoma binding protein 6 (RBBP6) is a 250 kDa RING finger-containing protein whose function is known to be mediated through interaction with other proteins. RBBP6 plays a role in the regulation of the tumour ... -
The structure of the nitrilase from Rhodococcus Rhodochrous J1: homology modeling and three-dimensional reconstruction
(University of the Western Cape, 2006)The nitrilases are an important class of industrial enzymes that are found in all phyla. These enzymes are expressed widely in prokaryotes and eukaryotes. Nitrilases convert nitriles to corresponding acids and ammonia. ... -
The structure, function and engineering of a thermostable nitrile hydratase
(University of the Western Cape, 2008)Nitrile hydratases (NHases) are enzymes that catalyse the conversion of organocyanides to amides via a non-hydrolytic hydration reaction. They are industrially relevant enzymes, currently used in the manufacture of ... -
A study of the problems and possibilities of using the marine intertidal zone for teaching principles of ecology in senior secondary schools: A survey of biology teachers in the western cape.
(University of the Western Cape, 1994)In this study I investigate the attitudes of a group biology teachers in the Western Cape, to using the marine intertidal zone for teaching principles of ecology in senior secondary schools, by exploring the problems ... -
Synthesis of silver doped titanium dioxide nanocomposites using tea extract from Aspalathus linearis and evaluation of their antibacterial effects.
(University of the Western Cape, 2018)Despite the wide success of antimicrobial agents against waterborne pathogens, waterborne disease continues to pose a threat to both mankind and animals. A major concern is that certain bacteria have developed resistance ... -
Synthesis of silver nanoparticles and investigating their antimicrobial effects
(University of the Western Cape, 2015)Water is essential for life, yet access to safe drinking water is still a major concern worldwide due to waterborne diseases. The current study proposes silver nanoparticles (AgNPs) as an antibacterial agent. Silver ...