Browsing Department of Biotechnology by Title
Now showing items 48-67 of 360
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Characterization of the role of a cycloartenol synthase gene (ZMCAS494) in Zea mays responses to drought stress
(University of the Western Cape, 2016)Plant Sterols are an important part of the membrane and their role is to control cell membrane fluidity and permeability. Several studies have indicated that plants that are mutant for genes that play a role in the sterol ... -
Characterization of the role of single domain soybean cystatins in regulating drought responses in soybean
(University of the Western Cape, 2015)This study investigated the effects that drought stress imposed on the growth and development of soybean plants. Soybeans were initially observed at the whole-plant level in order to identify the physical changes that had ... -
Characterization of the role of Zea mays burp domain-containing genes in maize drought responses
(University of the Western Cape, 2016)Global climate change has resulted in altered rainfall patterns, causing annual losses in maize crop yield due to water deficit stress. Therefore, it is important to produce maize cultivars which are more drought-tolerant. ... -
Characterization of two Arabidopsis thaliana genes with roles in plant homeostasis
(University of the Western Cape, 2004)Plants are continuously exposed to varying conditions in their environment, to which they have to adapt by manipulating various cellular processes. Environmental (abiotic) and pathogen (biotic) stress are challenges against ... -
Cloning and characterization of three compost metagenome-derived α‑L‑Arabinofuranosidases with differing thermal stabilities
(University of the Western Cape, 2014)Second generation biofuels production requires a suite of lignocellulolytic enzymes, acting synergistically to liberate the fermentable monosaccharides contained within agricultural waste materials. The use of thermostable ... -
Cloning, expression and characterisation of Amidase Genes from a psychrotolerant Nesterenkonia isolate
(University of the Western Cape, 2009)A nitrile and amide hydrolysing Nesterenkonia sp. was isolated from Antarctic soil and was characterised as a psychrotolerant, halotolerant and alkaliphilic extremophile. Amidases are widely distributed in both prokaryotic ... -
Cloning, expression and characterization of Novel Lipase and Esterases from Burkholderia multivorans UWC10
(2005)An esterase and lipase producing Burkholderia multivorans strain was isolated by culture enrichment strategies. A shotgun library of Burkholderia multivorans genomic DNA (prepared in E. coli/pUC18) was screened for lipase ... -
Comparative analysis of 3, 3'- diindolylmethane and indole-3-carbinol respective treatments in Brassica napus L.
(University of the Western Cape, 2017)Lately, there has been serious contamination of soils and ground water caused by mineral mining and environmental pollution leading to heavy metal accumulation within soils and over time rendering the soil infertile. The ... -
Comparative analysis of molecular and physiological responses of two canola genotypes to drought stress
(University of the Western Cape, 2016)Food security has always been one of the priority concerns in Africa, and it is mostly threatened by drought stress due to climate change. Drought-induced stress is one of the serious limiting factors of plant production, ... -
Comparative analysis of sorghum and other South African grains for sustainable bioethanol production
(University of the Western Cape, 2012)The depletion of oil reserves and the constant discharge of greenhouse gasses (GHG) that are associated with global warming have forced both political and scientific sectors to pursue alternative, renewable and sustainable ... -
Comparative analysis of sugar-biosynthesis proteins of sorghum stems and the investigation of their role in hyperosmotic stress tolerance
(University of the Western Cape, 2015)Sorghum bicolor (L.) Moench is an important cereal crop currently explored as a potential bio-energy crop due to its stress tolerance and ability to ferment soluble sugars. Physiological studies on sorghum varieties have ... -
A comparative ancestry analysis of Y-chromosome DNA haplogroups using high resolution melting
(University of the Western Cape, 2018)The objective of this study is to deduce paternal ancestry using ancestry informative single nucleotide polymorphisms (SNPs) by means of High Resolution Melting (HRM). This was completed by producing a multiplex system that ... -
Comparative molecular, physiological and proteomic analyses of maize and sorghum subjected to water deficit stress
(University of the Western Cape, 2019)Drought is a major abiotic stress which causes not only differences between the mean yield and the potential yield but also yield variation from year to year. Although selection for genotypes with improved productivity ... -
A comparative proteomic analysis of two contrasting Salvia hispanica L. genotypes under salinity stress
(University of the Western cape, 2016)Salvia hispanica L. is an annual pseudocereal food crop, locally known as chia that has the ability to grow in water stress environments. The importance of chia dates back to the pre-columbian era where it was consumed as ... -
Comparison of actinobacterial diversity in Marion Island terrestrial habitats
(University of the Western Cape, 2008)The major aim of this study is to determine and compare the distribution of bacteria and actinobacteria in Marion Island terrestrial habitats. -
Comparison of multi-gene integration strategies in CRISPR-based transformation of Saccharomyces cerevisiae
(University of Western Cape, 2021)Saccharomyces cerevisiae is an important host in industrial biotechnology. This yeast is the host of choice for the first and second-generation biofuels for ethanol production. Genome modification in S. cerevisiae has been ... -
Comparison of multi-gene integration strategies in CRISPR-based transformation of Saccharomyces cerevisiae
(University of the Western Cape, 2021)Saccharomyces cerevisiae is an important host in industrial biotechnology. This yeast is the host of choice for the first and second-generation biofuels for ethanol production. Genome modification in S. cerevisiae has been ... -
Comparison of plasmids from clinical Lactobacillus strains
(University of the Western Cape, 2018)The vaginal mucosa is dominated by Gram positive, rod shaped lactobacilli which serve as a natural barrier against infection. In both healthy and BV infected women Lactobacillus crispatus and Lactobacillus jensennii has ... -
Comparison of plasmids from clinical Lactobacillus strains
(University of the Western Cape, 2018)The vaginal mucosa is dominated by Gram positive, rod shaped lactobacilli which serve as a natural barrier against infection. In both healthy and BV infected women Lactobacillus crispatus and Lactobacillus jensennii has ... -
A computational framework for transcriptome assembly and annotation in non-model organisms: the case of venturia inaequalis
(University of the Western Cape, 2014)In this dissertation three computational approaches are presented that enable optimization of reference-free transcriptome reconstruction. The first addresses the selection of bona fide reconstructed transcribed fragments ...