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The structure of the nitrilase from Rhodococcus Rhodochrous J1: homology modeling and three-dimensional reconstruction
(University of the Western Cape, 2006)
The nitrilases are an important class of industrial enzymes that are found in all phyla. These enzymes are expressed widely in prokaryotes and eukaryotes. Nitrilases convert nitriles to corresponding acids and ammonia. ...
Structure, enzymology and genetic engineering of Bacillus sp. RAPc8 nitrile hydratase
(University of the Western Cape, 2005)
Microbial nitrile hydratases (NHases) are important industrial enzymes that catalyse the conversion of nitriles to the corresponding amides. A thermostable, cobalt-type
Bacillus sp. RAPc8 NHase was previously cloned and ...
Recombinant expression of the pRb- and p53-interacting domains from the human RBBP6 protein for in vitro binding studies
(University of the Western Cape, 2004)
This thesis describes the cloning and recombinant expression of domains from the human RBBP6 protein for future in vitro binding studies with pRb and p53. RBBP6 is a splicing-associated protein that is known to interact ...
Comparison of multi-gene integration strategies in CRISPR-based transformation of Saccharomyces cerevisiae
(University of the Western Cape, 2021)
Saccharomyces cerevisiae is an important host in industrial biotechnology. This yeast is the host of choice for the first and second-generation biofuels for ethanol production. Genome modification in S. cerevisiae has been ...
Biocontrol potential of fungal endophytes against Fusarium proliferatum in maize
(University of the Western Cape, 2022)
Fusarium proliferatum is a fungal pathogen that is the cause of numerous diseases in various
crops of fruit and vegetables. About 25% of the maize crops harvested annually are affected by
mycotoxins produced by F. ...
Identification of sub-clinical biomarkers that predict the risk of developing diabetic cardiomyopathy
(University of the Western Cape, 2022)
Cardiovascular disease (CVD) is the leading cause of death of people with obesity and type 2
diabetes (T2DM). According to a statistical report from the World Health Organization
(WHO), approximately 17.9 million people ...
The effect of chitosan in the germination and growth of sorghum bicolor under salt stress
(University of the Western Cape, 2022)
The agricultural sector has been facing enormous challenges including reduced crop yield and increased food production demand to cater for the growing population. Crop production is severely affected by abiotic and biotic ...
The interactive effects of salt stress and Fusarium proliferatum infection on maize seedlings
(University of the Western Cape, 2022)
Field crops are often subjected to multiple co-occurring stress factors, and they evolved specific mechanisms to counteract the effects of these stress factors. Most studies explore the fundamental molecular mechanisms ...
Inter-individual genetic variation and the development of hypertension in a Xhosa African population of Eastern Cape, South Africa
(University of the Western Cape, 2022)
Cardiovascular diseases (CVD) are the leading cause of death globally, accounting for 18.6 million deaths. Hypertension (HTN) drives the global burden of CVD and is a leading cause of cardiovascular-related mortality with ...
Exogenous carbon monoxide and jasmonic acid mitigate salt stress in Sorghum bicolor
(University of the Western Cape, 2022)
The agricultural sector plays an important role in the world economy. Expanding research is
piloting its improved contribution to the economic sector. Despite the economic gain, the
increasing population has already put ...