Bioinformatics and polymerase chain reaction: Tools to determine the host specificity of Salmonella typhi murium

Abstract

The nucleotide primer pair, Redl and Red2, was designed from the nucleotide sequence obtained from the NCBI database (Fig. 2.1). This nucleotide sequence encodes for the Salmonella typhimurium invasion gene D protein (sigD) and invasion gene E protein (sigE) genes. The Sa/monella and the non-Salmonella serovars used were subjected to PCR conditions at various annealing temperatures (T.) (Tables 2.4, 2.5,2.6). This was performed in order to optimize the PCR. Plasmid DNA PCR amplicons (350bp) detected S.braenderup and S.gallinarum at 53oC (Fig. 2.7) and at 56oC, S.braenderup at 54oC and S.gatlinarum at 55oC. PCR of the plasmid DNA did however not detect Salmonella typhimurium, the serotype it was designed to detec